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Optimizing large organ scale micro computed tomography imaging in pig and human hearts using a novel air-drying technique

hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorPALLARES-LUPON, N.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorRAMLUGUN, G.
hal.structure.identifierIHU-LIRYC
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorOZENNE, Valéry
hal.structure.identifierIHU-LIRYC
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorDUCHÂTEAU, J.
hal.structure.identifierIHU-LIRYC
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorDELGOVE, A.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorBAYER, J.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorMORENO, A.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorCONSTANTIN, M.
hal.structure.identifierUniversity of Auckland [Auckland]
dc.contributor.authorGERNEKE, D.
hal.structure.identifierUniversity of Auckland [Auckland]
dc.contributor.authorSANDS, G.B.
hal.structure.identifierUniversity of Auckland [Auckland]
dc.contributor.authorTREW, M.L.
hal.structure.identifierIHU-LIRYC
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorHOCINI, M.
hal.structure.identifierIHU-LIRYC
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorHAISSAGUERRE, M.
hal.structure.identifierIHU-LIRYC
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
hal.structure.identifierUniversity of Calgary
dc.contributor.authorVIGMOND, E.J.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorQUESSON, B.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorBERNUS, O.
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierIHU-LIRYC
dc.contributor.authorWALTON, Richard
dc.date.accessioned2024-04-04T02:40:07Z
dc.date.available2024-04-04T02:40:07Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/191037
dc.description.abstractEnAbstract Underlying electrical propagation in the heart and potentially fatal arrhythmia is the cardiac microstructure. Despite the critical role of muscle architecture, a non-destructive approach to examine not only myocyte orientation, but cellular arrangement in to laminar organization is lacking in hearts from translational animal models and humans. X-ray micro computed tomography using contrast enhancing agents achieves three-dimensional images at near-histological resolutions. However, imaging large mammalian hearts presents challenges including X-ray over-attenuation and loss of image contrast. The goal of this study was to rethink tissue pre-treatment to optimize, and benefit from micro computed tomography imaging resolution in large tissues. Whole pig and human hearts were dehydrated and perfused with a tissue reinforcing agent, hexamethyldisilazane, and slowly air-dried. Heart morphology was conserved and temporally stable. This enabled direct air-mounting for micro computed tomography imaging. Moreover, the desiccated tissue density was significantly reduced compared to the initial hydrated state (P=0.04). Three-dimensional image reconstructions of air-dried hearts segmented using a single intensity threshold revealed detailed microstructural architecture of myolaminae. Conversely, one-step segmentation of hearts loaded with contrast agents poorly estimated the gross anatomical morphology of the heart and lacked identification of tissue microarchitecture. Air-drying large mammalian hearts optimizes X-ray imaging of cardiac microstructure.
dc.language.isoen
dc.title.enOptimizing large organ scale micro computed tomography imaging in pig and human hearts using a novel air-drying technique
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.1101/2021.07.29.454121
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
hal.identifierhal-03809841
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03809841v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=PALLARES-LUPON,%20N.&RAMLUGUN,%20G.&OZENNE,%20Val%C3%A9ry&DUCH%C3%82TEAU,%20J.&DELGOVE,%20A.&rft.genre=preprint


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