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Transcriptomic response of the benthic freshwater diatom Nitzschia palea exposed to Few Layer Graphene

dc.rights.licenseopenen_US
hal.structure.identifierLaboratoire Ecologie Fonctionnelle et Environnement [LEFE]
dc.contributor.authorGARACCI, Marion
hal.structure.identifierLaboratoire Ecologie Fonctionnelle et Environnement [LEFE]
dc.contributor.authorBARRET, Maialen
hal.structure.identifierGénomique et Biotechnologie des Fruits [GBF]
dc.contributor.authorFOLGOAS, Clément
hal.structure.identifierCentre interuniversitaire de recherche et d'ingénierie des matériaux [CIRIMAT]
dc.contributor.authorFLAHAUT, Emmanuel
hal.structure.identifierCentre interuniversitaire de recherche et d'ingénierie des matériaux [CIRIMAT]
dc.contributor.authorCHIMOWA, George
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBERTUCCI, Anthony
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierLaboratoire Ecologie Fonctionnelle et Environnement [LEFE]
dc.contributor.authorSILVESTRE, Jérôme
hal.structure.identifierLaboratoire Ecologie Fonctionnelle et Environnement [LEFE]
dc.contributor.authorGAUTHIER, Laury
hal.structure.identifierGénomique et Biotechnologie des Fruits [GBF]
dc.contributor.authorZOUINE, Mohamed
hal.structure.identifierLaboratoire Ecologie Fonctionnelle et Environnement [LEFE]
dc.contributor.authorPINELLI, Eric
dc.date.accessioned2024-02-29T08:41:32Z
dc.date.available2024-02-29T08:41:32Z
dc.date.issued2019
dc.identifier.issn2051-8153en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/188500
dc.description.abstractEnNanotechnology currently undergoes a rising development partly due to the increasing use of carbon-based nanoparticles, such as Few Layer Graphene (FLG). Owing to their numerous applications, their industrial production is likely to lead to environmental release, including into aquatic ecosystems. In this study, a transcriptomic approach was used to assess the effect of FLG at low (0.1 mg.L-1) and high (50 mg.L-1) concentration on the benthic freshwater diatom Nitzschia palea after 48h of exposure. Direct contact with FLG and induced shading were distinguished to compare the transcriptomic responses. Differentially expressed genes between each exposure and control conditions were identified and their functional description was discussed. Slight transcriptomic response related to cell wall repair was observed in diatoms exposed to low FLG concentration. Exposure to high FLG concentration induced a strong response involving 1907 transcripts. Notably, 16 transcripts involved in chlorophyll biosynthesis process were under-expressed (Log2FoldChange between -3 and -1.2),suggesting a down-regulation of photosynthetic metabolism. Diatoms exposed to high FLG concentration over-expressed about 13 transcripts encoding for extracellular proteins playing a role in cellular adhesion, and two highly up-regulated transcripts involved in cell wall repair. Light deprivation caused by shading induced a down-regulation of genes involved in the energetic metabolism of N. palea. Overall, these results revealed that metabolic pathways impacted by FLG exposure were concentration contact-dependent. Moreover, this study suggests that low FLG concentration, close to environmental conditions, will have minor impact on diatom biofilms whereas high FLG concentration, mimicking accidental release, might be critical for ecosystems.
dc.language.isoENen_US
dc.subject.enEcosystem
dc.subject.enNanotechnology
dc.title.enTranscriptomic response of the benthic freshwater diatom Nitzschia palea exposed to Few Layer Graphene
dc.typeArticle de revueen_US
dc.identifier.doi10.1039/C8EN00987Ben_US
dc.subject.halSciences du Vivant [q-bio]/Toxicologie/Ecotoxicologieen_US
dc.subject.halSciences de l'environnement/Biodiversité et Ecologieen_US
bordeaux.journalEnvironmental science‎.Nanoen_US
bordeaux.page1363-1381en_US
bordeaux.volume6en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue5en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-02160439
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental%20science%E2%80%8E.Nano&rft.date=2019&rft.volume=6&rft.issue=5&rft.spage=1363-1381&rft.epage=1363-1381&rft.eissn=2051-8153&rft.issn=2051-8153&rft.au=GARACCI,%20Marion&BARRET,%20Maialen&FOLGOAS,%20Cl%C3%A9ment&FLAHAUT,%20Emmanuel&CHIMOWA,%20George&rft.genre=article


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