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Comparative genomics of Mycoplasma feriruminatoris , a fast-growing pathogen of wild Caprinae

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBABY, Vincent
hal.structure.identifierMycoplasmoses animales – UMR [MYCO]
dc.contributor.authorAMBROSET, Chloé
hal.structure.identifierMycoplasmoses animales – UMR [MYCO]
dc.contributor.authorGAURIVAUD, Patrice
hal.structure.identifierUniversité de Fribourg = University of Fribourg [UNIFR]
dc.contributor.authorFALQUET, Laurent
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBOURY, Christophe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorGUICHOUX, Erwan
hal.structure.identifierUniversität Bern / University of Bern [UNIBE]
dc.contributor.authorJORES, Joerg
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierMycoplasmoses animales – UMR [MYCO]
dc.contributor.authorTARDY, Florence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
dc.date.issued2023-03-21
dc.description.abstractEnMycoplasma feriruminatoris is a fast-growing Mycoplasma species isolated from wild Caprinae and first described in 2013. M. feriruminatoris isolates have been associated with arthritis, keratoconjunctivitis, pneumonia and septicemia, but were also recovered from apparently healthy animals. To better understand what defines this species, we performed a genomic survey on 14 strains collected from free-ranging or zoo-housed animals between 1987 and 2017. The average chromosome size of the M. feriruminatoris strains was 1,040 ± 0,024 kbp, with 24% G+C and 852 ± 31 CDS. The core genome and pan-genome of the M. feriruminatoris species contained 628 and 1,312 protein families, respectively. The M. feriruminatoris strains displayed a relatively closed pan-genome, with many features and putative virulence factors shared with species from the M. mycoides cluster, including the MIB-MIP Ig cleavage system, a repertoire of DUF285 surface proteins and a complete biosynthetic pathway for galactan. M. feriruminatoris genomes were found to be mostly syntenic, although repertoires of mobile genetic elements, including Mycoplasma Integrative and Conjugative Elements, insertion sequences, and a single plasmid varied. Phylogenetic- and gene content analyzes confirmed that M. feriruminatoris was closer to the M. mycoides cluster than to the ruminant species M. yeatsii and M. putrefaciens . Ancestral genome reconstruction showed that the emergence of the M. feriruminatoris species was associated with the gain of 17 gene families, some of which encode defense enzymes and surface proteins, and the loss of 25 others, some of which are involved in sugar transport and metabolism. This comparative study suggests that the M. mycoides cluster could be extended to include M. feriruminatoris . We also find evidence that the specific organization and structure of the DnaA boxes around the oriC of M. feriruminatoris may contribute to drive the remarkable fast growth of this minimal bacterium.
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.title.enComparative genomics of Mycoplasma feriruminatoris , a fast-growing pathogen of wild Caprinae
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.1101/2023.03.21.533155
dc.subject.halSciences du Vivant [q-bio]/Génétique
hal.identifierhal-04172347
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04172347v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2023-03-21&rft.au=BABY,%20Vincent&AMBROSET,%20Chlo%C3%A9&GAURIVAUD,%20Patrice&FALQUET,%20Laurent&BOURY,%20Christophe&rft.genre=preprint


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