Afficher la notice abrégée

Identification and characterization of water-stress-responsive genes in hydroponically grown maritime pine (Pinus pinaster) seedlings

hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorDUBOS, Christian
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorLE PROVOST, Grégoire
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorPOT, David
hal.structure.identifierLaboratoire de Chimie des Substances Végétales
dc.contributor.authorSALIN, Franck
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorMADUR, Delphine
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorFRIGERIO, Jean-Marc
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.issued2003-01-31
dc.identifier.issn0829-318X
dc.description.abstractEnGrowth, development and productivity of longlived organisms such as forest trees are continuously challenged by abiotic stresses, and may also be greatly affected by predicted climatic change. As a first step toward creating stress-resistant maritime pine (Pinus pinaster Ait.) varieties by marker-assisted breeding, we describe the identification and characterization of water-stress-responsive genes in hydroponically grown seedlings that were well watered (-0.08 MPa) or subjected to water deprivation (-0.45 MPa) by the addition of polyethylene glycol. The cDNA amplified fragment-length polymorphism (cDNA-AFLP) technique was used to identify genes regulated by water deprivation. Approximately 4000 transcript-derived fragments (TDFs) were screened, of which 28 increased and 20 decreased in seedlings subjected to water deprivation. Of these 48 TDFs, 62.6% corresponded to proteins of known function, which indicate the main mechanisms involved in the osmotic stress response (photosynthesis, carbohydrate metabolism, cell wall synthesis and plant defense). We found that 16.6% of the 48 TDFs were similar to Arabidopsis thaliana (L.) Heynh gene products, 10.4% were similar to Pinus taeda L. expressed sequence tags (ESTs) and 10.4% did not match any sequences in the public databases. The relative abundance of these transcripts was quantitatively analyzed by reverse northern of both needle and root tissues, confirming the effectiveness of the cDNA-AFLP technique in detecting differentially expressed genes. The identification and characterization of water-stress-responsive genes provide new insights into the nature of the machinery involved in the response to water deprivation in a forest tree.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.encDNA-AFLP
dc.subject.enosmotic stress
dc.subject.enpolyethylene glycol
dc.subject.enreverse northern
dc.title.enIdentification and characterization of water-stress-responsive genes in hydroponically grown maritime pine (Pinus pinaster) seedlings
dc.typeArticle de revue
dc.identifier.doi10.1093/treephys/23.3.169
dc.subject.halSciences de l'environnement
bordeaux.journalTree Physiology
bordeaux.page169-179
bordeaux.volume23
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-04200586
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04200586v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Tree%20Physiology&rft.date=2003-01-31&rft.volume=23&rft.issue=3&rft.spage=169-179&rft.epage=169-179&rft.eissn=0829-318X&rft.issn=0829-318X&rft.au=DUBOS,%20Christian&LE%20PROVOST,%20Gr%C3%A9goire&POT,%20David&SALIN,%20Franck&LALANNE,%20C%C3%A9line&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée