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Phaseolus vulgaris alphaendornavirus-1 is frequent in bean germplasm in Slovakia and shows low molecular variability

hal.structure.identifierSs. Cyril and Methodius University in Skopje
hal.structure.identifierNational Agriculture and Food Centre – Research Institute of Plant Production
dc.contributor.authorMRKVOVÁ, Michaela
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorACHS, Adam
hal.structure.identifierSs. Cyril and Methodius University in Skopje
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorALAXIN, Peter
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorŠUBR, Zdeno
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorPREDAJŇA, Lukáš
hal.structure.identifierNational Agriculture and Food Centre – Research Institute of Plant Production
dc.contributor.authorZETOCHOVÁ, Erika
hal.structure.identifierNational Agriculture and Food Centre – Research Institute of Plant Production
dc.contributor.authorHAUPTVOGEL, Pavol
hal.structure.identifierComenius University in Bratislava
dc.contributor.authorŠOLTYS, Katarína
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierSs. Cyril and Methodius University in Skopje
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorGLASA, Miroslav
dc.date.accessioned2023-11-20T17:18:22Z
dc.date.available2023-11-20T17:18:22Z
dc.date.issued2023-06-05
dc.identifier.issn0001-723X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/185363
dc.description.abstractEnPhaseolus vulgaris alphaendornavirus-1 (PvEV-1, family Endornaviridae) was identified by ribodepleted total RNA high-throughput sequencing in the virome of two bean plants (Phaseouls vulgaris L.) grown in a garden in western Slovakia. Two nearly complete PvEV-1 genomes (ca. 14.06 kb, named PV1 and PV2) were assembled, showing 99.9% nucleotide identity, while their nucleotide identity with the reference PvEV-1 genome (NC_039217) reached 98.4%. Two primer pairs spanning the viral helicase encoding region and sequence upstream of the RNA-dependent RNA polymerase were designed and used to confirm the presence of the virus in the original bean samples by RT-PCR. A subsequent search for PvEV-1 presence in Slovakia was focused on two groups of samples: 1) bean plants grown under open field conditions and sampled during the vegetation period and 2) bean accessions grown from seeds obtained from a Slovak and French bean germplasm collection. Based on RT-PCR results, 4 out of 15 bean samples from open fields and 12 out of 21 bean accessions from the curated germplasm collection tested PvEV-1-positive. Interestingly, sequencing of RT-PCR products revealed that all amplified isolates are identical in the two amplified genomic portion which is also identical to those of the PV1 and PV2 isolates. These results suggest a relatively high incidence of PvEV-1 in bean in Slovakia. This is the first evidence and characterization of PvEV-1 from bean plants in Europe.
dc.language.isoen
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enbean
dc.subject.enEndornaviridae
dc.subject.engenotype
dc.subject.enPvEV-1
dc.subject.envertical transmission
dc.title.enPhaseolus vulgaris alphaendornavirus-1 is frequent in bean germplasm in Slovakia and shows low molecular variability
dc.typeArticle de revue
dc.identifier.doi10.3389/av.2023.11484
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalActa Virologica
bordeaux.volume67
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-04121937
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04121937v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Acta%20Virologica&rft.date=2023-06-05&rft.volume=67&rft.eissn=0001-723X&rft.issn=0001-723X&rft.au=MRKVOV%C3%81,%20Michaela&ACHS,%20Adam&ALAXIN,%20Peter&%C5%A0UBR,%20Zdeno&PREDAJ%C5%87A,%20Luk%C3%A1%C5%A1&rft.genre=article


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