Mostrar el registro sencillo del ítem
A diffusion cell adapted to nuclear imaging instruments for the measurement of molecular release and pharmacokinetics across membranes
| dc.rights.license | open | en_US |
| dc.contributor.author | OMAR, Mahmoud Mohamed | |
| dc.contributor.author | LAPRISE-PELLETIER, Myriam | |
| dc.contributor.author | LEMAY, Sophie | |
| dc.contributor.author | LAGUEUX, Jean | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | TUDURI, Ludovic | |
| dc.contributor.author | FORTIN, Marc-André | |
| dc.date.accessioned | 2023-11-20T10:50:53Z | |
| dc.date.available | 2023-11-20T10:50:53Z | |
| dc.date.issued | 2021-09-10 | |
| dc.identifier.issn | 0168-3659 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184845 | |
| dc.description.abstractEn | Diffusion cells are routinely used in pharmacology to measure the permeation of pharmaceutical compounds and contaminants across membranes (biological or synthetic). They can also be used to study drug release from excipients. The device is made of a donor (DC) and an acceptor (AC) compartment, separated by a membrane. Usually, permeation of molecules across membranes is measured by sampling from the AC at different time points. However, this process disturbs the equilibrium of the cell. Furthermore, analytical techniques used in association with diffusion cells sometimes lack either accuracy, sensitivity, or both. This work reports on the development of nuclear imaging – compatible diffusion cells. The cell is made of a polymer transparent to high-energy photons typically detected in positron emission tomography (PET). It was tested in a finite-dose set-up experiment with a pre-clinical PET system. Porous cellulose membranes (3.5, 25 and 300 kDa), a common excipient in pharmacology, as well as for dialysis membranes, were used as test membranes. The radioisotope 89Zr chelated with deferoxamine B (DFO; 0.65 kDa), was used as an imaging probe (7–10 MBq; 0.2–0.3 nMol 89Zr-DFO). In medicine, DFO is also commonly used for iron removal treatments and pharmacological formulations often require the association of this molecule with cellulose. Permeation profiles were obtained by measuring the radioactivity in the DC and AC for up to 2 weeks. The kinetic profiles were used to extract lag time, influx, and diffusion coefficients of DFO across porous cellulose membranes. A sensitivity threshold of 0.005 MBq, or 3.4 fmol of 89Zr-DFO, was revealed. The lag time to permeation (τ) measured in the AC compartment, was found to be 1.33, 0.5, and 0.19 h with 3.5, 25, and 300 kDa membranes, respectively. Diffusion coefficients of 3.65 × 10−6, 8.33 × 10−6, and 4.74 × 10−5 cm2 h−1 where revealed, with maximal pseudo steady-state influx values (Jpss) of 6.55 × 10−6, 1.76 × 10−5, and 1.29 × 10−5 nmol cm−2 h−1. This study confirms the potential of the technology for monitoring molecular diffusion and release processes at low concentrations, high sensitivities, in real time and in a visual manner. © 2021 Elsevier B.V. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Amines | |
| dc.subject.en | Analytic Method | |
| dc.subject.en | Article | |
| dc.subject.en | Cell-Be | |
| dc.subject.en | Cells | |
| dc.subject.en | Cellulose | |
| dc.subject.en | Chelation | |
| dc.subject.en | Controlled Drug Delivery | |
| dc.subject.en | Controlled Study | |
| dc.subject.en | Cytology | |
| dc.subject.en | Deferoxamine | |
| dc.subject.en | Dialysis Membranes | |
| dc.subject.en | Diffusion Cells | |
| dc.subject.en | Diffusion Coefficient | |
| dc.subject.en | Diffusion Measurements | |
| dc.subject.en | Drug Release | |
| dc.subject.en | Emission Tomography | |
| dc.subject.en | Excipient | |
| dc.subject.en | Hemodialysis | |
| dc.subject.en | Iron | |
| dc.subject.en | Kinetic Parameters | |
| dc.subject.en | Mathematical Model | |
| dc.subject.en | Membrane Permeability | |
| dc.subject.en | Membrane Transport | |
| dc.subject.en | Molecular Biology | |
| dc.subject.en | Molecular Interaction | |
| dc.subject.en | Molecular Release | |
| dc.subject.en | Molecules | |
| dc.subject.en | Nuclear Imaging | |
| dc.subject.en | Partition Coefficient | |
| dc.subject.en | Permeation Measurements | |
| dc.subject.en | Pharmacokinetic Parameters | |
| dc.subject.en | Photon | |
| dc.subject.en | Polymer Membrane | |
| dc.subject.en | Positron Annihilation Spectroscopy | |
| dc.subject.en | Positron Emission Tomography | |
| dc.subject.en | Positrons | |
| dc.subject.en | Radioactivity | |
| dc.subject.en | Radioisotopes | |
| dc.subject.en | Renal Dialysis | |
| dc.subject.en | Steady State | |
| dc.subject.en | Targeted Drug Delivery | |
| dc.subject.en | Tissue Distribution | |
| dc.subject.en | Zirconium | |
| dc.title.en | A diffusion cell adapted to nuclear imaging instruments for the measurement of molecular release and pharmacokinetics across membranes | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.jconrel.2021.07.013 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| bordeaux.journal | Journal of Controlled Release | en_US |
| bordeaux.page | 661-675 | en_US |
| bordeaux.volume | 337 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | LPTC | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-04295165 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-20T10:50:55Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Controlled%20Release&rft.date=2021-09-10&rft.volume=337&rft.spage=661-675&rft.epage=661-675&rft.eissn=0168-3659&rft.issn=0168-3659&rft.au=OMAR,%20Mahmoud%20Mohamed&LAPRISE-PELLETIER,%20Myriam&LEMAY,%20Sophie&LAGUEUX,%20Jean&TUDURI,%20Ludovic&rft.genre=article |
Archivos en el ítem
| Archivos | Tamaño | Formato | Ver |
|---|---|---|---|
|
No hay archivos asociados a este ítem. |
|||