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Short-term metallothionein inductions in the edible cockle Cerastoderma edule after cadmium or mercury exposure: Discrepancy between mRNA and protein responses

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPAUL-PONT, Ika
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
IDREF: 105696722
dc.contributor.authorNILI, Hanane
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDE MONTAUDOUIN, Xavier
IDREF: 075931664
dc.date.accessioned2023-10-16T12:23:43Z
dc.date.available2023-10-16T12:23:43Z
dc.date.issued2010-05-01
dc.identifier.issn0166-445Xen_US
dc.identifier.urihttps://www.researchgate.net/publication/40833299_Short-term_metallothionein_inductions_in_the_edible_cockle_Cerastoderma_edule_after_cadmium_or_mercury_exposure_Discrepancy_between_mRNA_and_protein_responses
dc.identifier.urioai:crossref.org:10.1016/j.aquatox.2009.12.007
dc.identifier.urioai:researchgate.net:40833299
dc.identifier.uri32941298
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184438
dc.description.abstractEnMetallothioneins (MT) are essential metal binding proteins involved in metal homeostasis and detoxification in living organisms. Numerous studies have focused on MT response to metal exposure and showed an important variability according to species, metal, concentration and time of exposure. In this study, the expression of one isoform of MT gene (Cemt1) and associated MT protein synthesis were determined after 1, 3, 9, 24, 72 and 168h of cadmium (Cd) or mercury (Hg) exposures in gills of the cockle Cerastoderma edule. This experiment, carried out in laboratory conditions, revealed that in Cd-exposed cockles, induction of Cemt1 is time-dependent following a "pulse-scheme" with significant upregulation at 24h and 168h intersected by time point (72h) with significant downregulation. MT protein concentration increases with time in gills of exposed cockles in relation with the progressive accumulation of Cd in soluble fraction. On contrary, Hg exposure does not lead to any induction of Cemt1 mRNA expression or MT protein synthesis compared to control, despite a higher accumulation of this metal in gills of cockles compared to Cd. The localization of Hg (85-90%) is in insoluble fraction, whereas MT was located in the cytoplasm of cells. This gives us a first clue to understand the inability of Hg to activate MT synthesis. However, other biochemical processes probably occur in gills of C. edule since the remaining soluble fraction of Hg exceeds MT sequestration ability. Finally, since one of the first main targets of metal toxicity in cells was the mitochondria, some genes involved in mitochondria metabolism were also analyzed in order to assess potential differences in cellular damages between two metal exposures. Indeed, until T(168), no impact on mitochondrial genes was shown following Hg exposure, despite the complete lack of MT response. This result indicated the presence of other effective cellular ligands which sequester the cytosolic fraction of this metal and consequently inhibit metal reactivity. Such competition mechanisms with other cytosolic ligands more sensitive to Hg were particularly argued in the discussion.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.sourceorcid
dc.title.enShort-term metallothionein inductions in the edible cockle Cerastoderma edule after cadmium or mercury exposure: Discrepancy between mRNA and protein responses
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.aquatox.2009.12.007en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalAquatic Toxicologyen_US
bordeaux.page260-267en_US
bordeaux.volume97en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamECOBIOCen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04244355
hal.version1
hal.date.transferred2023-10-16T12:23:45Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Aquatic%20Toxicology&rft.date=2010-05-01&rft.volume=97&rft.issue=3&rft.spage=260-267&rft.epage=260-267&rft.eissn=0166-445X&rft.issn=0166-445X&rft.au=PAUL-PONT,%20Ika&GONZALEZ,%20Patrice&BAUDRIMONT,%20Magalie&NILI,%20Hanane&DE%20MONTAUDOUIN,%20Xavier&rft.genre=article


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