Molecular confirmation of HIV-1 and HIV-2 coinfections among initially serologically dually-reactive samples from patients living in West Africa.
| dc.rights.license | open | en_US |
| dc.contributor.author | TCHOUNGA, Boris K | |
| dc.contributor.author | BERTINE, Mélanie | |
| dc.contributor.author | DAMOND, Florence | |
| dc.contributor.author | FERRÉ, Valentine Marie | |
| dc.contributor.author | INWOLEY, André | |
| dc.contributor.author | BONI, Simon P | |
| dc.contributor.author | MOISAN, Alice | |
| dc.contributor.author | PLANTIER, Jean-Christophe | |
| dc.contributor.author | DESCAMPS, Diane | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| hal.structure.identifier | Global Health in the Global South [GHiGS] | |
| dc.contributor.author | EKOUEVI, Didier Koumavi | |
| dc.contributor.author | CHARPENTIER, Charlotte | |
| dc.date.accessioned | 2023-10-04T13:49:05Z | |
| dc.date.available | 2023-10-04T13:49:05Z | |
| dc.date.issued | 2023-03-31 | |
| dc.identifier.issn | 1932-6203 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184320 | |
| dc.description.abstractEn | This study aimed to confirm the co-infection with HIV-1 and HIV-2, among West African patients using in-house HIV type/group enzyme-immuno assays and molecular diagnosis. A cross-sectional survey was conducted from April 2016 to October 2017 in the biggest HIV clinics of Côte d'Ivoire and Burkina Faso. A first serological confirmation was done in the referral laboratory using an in-house, indirect immuno-enzymatic essay allowing the qualitative detection of both HIV-1 and HIV-2 antibodies. In order to separately detect anti-HIV-1 and anti-HIV-2 antibodies, a type/group specific enzyme-immuno assay (HIV-GSEIA) was used. To confirm the co-infections, HIV-1 and HIV-2 DNA-qualitative PCR assays were performed. A total of 91 patients were enrolled in the study and provided blood sample for HIV type confirmatory testing including 13 (14.3%) HIV-2 mono-reactive and 78 (85.7%) HIV-1/HIV-2 dually-reactive based on the HIV testing National Algorithms. The first serological ELISA confirmatory test performed showed that 80 (78.9%) of the 91 participants were dually-reactive. The HIV-GSEIA performed on these 80 serum samples retrieve one 61 HIV-1/HIV-2 dually-reactive samples. HIV-1 and HIV-2 DNA PCR were performed on 54 of the 61 HIV-1/HIV-2 dually-reactive samples and 46 out of 61 (75.4%) samples were found HIV-1/HIV-2 coinfected. The contribution of type/group specific enzyme-immuno assay to accurately identify HIV-1/HIV-2 coinfections remain suboptimal, emphasizing the need for molecular diagnosis platforms in West Africa, to avail HIV DNA PCR test for the confirmation of HIV-1/HIV-2 co-infections. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Humans | |
| dc.subject.en | Cross-Sectional Studies | |
| dc.subject.en | Coinfection | |
| dc.subject.en | HIV Infections | |
| dc.subject.en | HIV Seropositivity | |
| dc.subject.en | HIV-1 | |
| dc.subject.en | HIV Antibodies | |
| dc.subject.en | Cote d'Ivoire | |
| dc.subject.en | HIV-2 | |
| dc.title.en | Molecular confirmation of HIV-1 and HIV-2 coinfections among initially serologically dually-reactive samples from patients living in West Africa. | |
| dc.title.alternative | PLoS One | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1371/journal.pone.0283602 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Santé publique et épidémiologie | en_US |
| dc.identifier.pubmed | 37000823 | en_US |
| bordeaux.journal | PLoS ONE | en_US |
| bordeaux.page | e0283602 | en_US |
| bordeaux.volume | 18 | en_US |
| bordeaux.hal.laboratories | Bordeaux Population Health Research Center (BPH) - UMR 1219 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.team | GHIGS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | European and Developing Countries Clinical Trials Partnership | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04228561 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-10-04T13:49:07Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
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