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NiONP-Induced Oxidative Stress and Mitochondrial Impairment in an In Vitro Pulmonary Vascular Cell Model Mimicking Endothelial Dysfunction
| dc.rights.license | open | en_US |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | GERMANDE, Ophelie | |
| dc.contributor.author | DUCRET, Thomas | |
| dc.contributor.author | QUIGNARD, Jean-Francois | |
| dc.contributor.author | DEWEIRDT, Juliette | |
| dc.contributor.author | FREUND-MICHEL, Véronique | |
| dc.contributor.author | ERRERA, Marie-Hélène | |
| dc.contributor.author | CARDOUAT, Guillaume | |
| dc.contributor.author | VACHER, Pierre | |
| dc.contributor.author | MULLER, Bernard | |
| dc.contributor.author | BERGER, Patrick
ORCID: 0000-0003-4702-0343 IDREF: 060717998 | |
| dc.contributor.author | GUIBERT, Christelle | |
| dc.contributor.author | BAUDRIMONT, Magalie
IDREF: 105696722 | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | BAUDRIMONT, Magalie
IDREF: 105696722 | |
| dc.contributor.author | BAUDRIMONT, Isabelle | |
| dc.date.accessioned | 2023-06-16T12:18:53Z | |
| dc.date.available | 2023-06-16T12:18:53Z | |
| dc.date.issued | 2022-04-26 | |
| dc.identifier.issn | 2076-3921 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182707 | |
| dc.description.abstractEn | The development and use of nanomaterials, especially of nickel oxide nanoparticles (NiONPs), is expected to provide many benefits but also has raised concerns about the potential human health risks. Inhaled NPs are known to exert deleterious cardiovascular side effects, including pulmonary hypertension. Consequently, patients with pulmonary hypertension (PH) could be at increased risk for morbidity. The objective of this study was to compare the toxic effects of NiONPs on human pulmonary artery endothelial cells (HPAEC) under physiological and pathological conditions. The study was conducted with an in vitro model mimicking the endothelial dysfunction observed in PH. HPAEC were cultured under physiological (static and normoxic) or pathological (20% cycle stretch and hypoxia) conditions and exposed to NiONPs (0.5–5 μg/cm2) for 4 or 24 h. The following endpoints were studied: (i) ROS production using CM-H2DCF-DA and MitoSOX probes, (ii) nitrite production by the Griess reaction, (iii) IL-6 secretion by ELISA, (iv) calcium signaling with a Fluo-4 AM probe, and (v) mitochondrial dysfunction with TMRM and MitoTracker probes. Our results evidenced that under pathological conditions, ROS and nitrite production, IL-6 secretions, calcium signaling, and mitochondria alterations increased compared to physiological conditions. Human exposure to NiONPs may be associated with adverse effects in vulnerable populations with cardiovascular risks. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | endothelial dysfunction; calcium | |
| dc.subject.en | cyclic stretch | |
| dc.subject.en | human pulmonary artery endothelial cells | |
| dc.subject.en | nickel oxide nanoparticles | |
| dc.subject.en | mitochondria alteration | |
| dc.subject.en | reactive oxygen species | |
| dc.title.en | NiONP-Induced Oxidative Stress and Mitochondrial Impairment in an In Vitro Pulmonary Vascular Cell Model Mimicking Endothelial Dysfunction | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/antiox11050847 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| bordeaux.journal | Antioxidants | en_US |
| bordeaux.volume | 11 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.issue | 5 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | EA | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.export | false | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Antioxidants&rft.date=2022-04-26&rft.volume=11&rft.issue=5&rft.eissn=2076-3921&rft.issn=2076-3921&rft.au=GERMANDE,%20Ophelie&DUCRET,%20Thomas&QUIGNARD,%20Jean-Francois&DEWEIRDT,%20Juliette&FREUND-MICHEL,%20V%C3%A9ronique&rft.genre=article |
