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hal.structure.identifierStructures et propriétés d'architectures moléculaire [SPRAM - UMR 5819]
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorCAMPILLO, Clément
hal.structure.identifierDéveloppement et évolution [DE]
dc.contributor.authorJERBER, Julie
hal.structure.identifierDéveloppement et évolution [DE]
dc.contributor.authorFISCH, Cathy
hal.structure.identifierCentre des Matériaux [CDM]
dc.contributor.authorSIMOES-BETBEDER, Maria
hal.structure.identifierDéveloppement et évolution [DE]
dc.contributor.authorDUPUIS-WILLIAMS, Pascale
hal.structure.identifierlp2n-04,lp2n-16
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorSYKES, C.
dc.date.accessioned2023-05-12T10:58:40Z
dc.date.available2023-05-12T10:58:40Z
dc.date.issued2012
dc.identifier.issn1367-2630
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181982
dc.description.abstractEnIn this paper we assess the role of the protein MKS1 (Meckel syndrome type 1) in the cortical membrane mechanics of the ciliated protist Paramecium. This protein is known to be crucial in the process of cilium formation, and we investigate its putative role in membrane-cytoskeleton attachment. Therefore, we compare cells where the gene coding for MKS1 is silenced to wild-type cells. We found that scanning electron microscopy observation of the cell surface reveals a cup-like structure in wild-type cells that is lost in silenced cells. Since this structure is based on the underlying cytoskeleton, one hypothesis to explain this observation is a disruption of membrane attachment to the cytoskeleton in the absence of MKS1 that should affect plasma membrane mechanics. We test this by probing the mechanics of wild-type and silenced cells by micropipette aspiration. Strikingly, we observe that, at the same aspiration pressure, the membrane of silenced cells is easily aspirated by the micropipette whereas that of wild-type cells enters only at a moderate velocity, an effect that suggests a detachment of the membrane from the underlying cytoskeleton in silenced cells. We quantify this detachment by measuring the deformation of the cell cortex and the rate of cell membrane entry in the micropipette. This study offers a new perspective for the characterization of membrane-cytoskeleton attachment in protists and paves the way for a better understanding of the role of membrane-cortex attachment in cilium formation.
dc.language.isoen
dc.publisherInstitute of Physics: Open Access Journals
dc.subject.enTissue engineering
dc.subject.enMembranes
dc.subject.enbilayers
dc.subject.enand vesicles
dc.subject.enElectron microscopy
dc.subject.enProteins
dc.subject.enPseudopods
dc.subject.enlamellipods
dc.subject.encilia
dc.subject.enand flagella
dc.title.enMechanics of membrane-cytoskeleton attachment in Paramecium
dc.typeArticle de revue
dc.identifier.doi10.1088/1367-2630/14/12/125016
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
bordeaux.journalNew Journal of Physics
bordeaux.page125016, 9 p.
bordeaux.volume14
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00814768
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00814768v1
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