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Dynamic SIM for high-speed imaging and optical sectioning in living samples

hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorVERMEULEN, Pierre
hal.structure.identifierInstitut d'Astrophysique de Paris [IAP]
dc.contributor.authorORIEUX, François
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorSEPULVEDA, Eduardo
hal.structure.identifierAnalyse d'images biologiques - Biological Image Analysis [BIA]
dc.contributor.authorOLIVO-MARIN, Jean-Christophe
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorFRAGOLA, Alexandra
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorLORIETTE, Vincent
dc.date.accessioned2023-05-12T10:52:08Z
dc.date.available2023-05-12T10:52:08Z
dc.date.conference2012-04-01
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181853
dc.description.abstractEnLive cell studies at the molecular level strongly depend on imaging performances. Structured illumination microscopy (SIM) brings a significant gain, as an increase of a factor 2 in lateral resolution allows observation of many new phenomena. However its current use for biological applications still requires major technical improvements in order to combine lateral super resolution with video rate imaging and optical sectioning of live samples. We will present a structured illuminated microscope by fringe projection together with an original and efficient reconstruction algorithm (Orieux 2011) that only requires 4 acquired images (instead of 9) to obtain a super-resolution one. Using this improvement and a sliding recombination of the raw images, it is possible to create super-resolution movies with aquarter of the information renewed in each reconstructed image. This unique approach allows realizing dynamic SIM movies of live samples with an increased temporal resolution compared to other structured illuminated microscopes.In order to observe phenomena several micrometers deep into a cell, we combine SIM with optical sectioning techniques. It is indeed possible to perform super-resolution with optical sectioning using 7 images (versus 15 for other SIMs) by allowing the zero order to interfere with the ±1 orders. We will present high spatio-temporal resolution observations of molecular mechanisms underlying transport intermediates biogenesis.
dc.language.isoen
dc.title.enDynamic SIM for high-speed imaging and optical sectioning in living samples
dc.typeCommunication dans un congrès avec actes
dc.subject.halInformatique [cs]/Traitement des images
dc.subject.halInformatique [cs]/Traitement du signal et de l'image
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
dc.subject.halStatistiques [stat]/Applications [stat.AP]
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.countrySG
bordeaux.title.proceedingFocus on Microscopy
bordeaux.conference.citySingapour
bordeaux.peerReviewedoui
hal.identifierhal-01623757
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01623757v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=VERMEULEN,%20Pierre&ORIEUX,%20Fran%C3%A7ois&SEPULVEDA,%20Eduardo&OLIVO-MARIN,%20Jean-Christophe&FRAGOLA,%20Alexandra&rft.genre=proceeding


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