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Complement in Human Pre-implantation Embryos: Attack and Defense

hal.structure.identifierUniversity of Oxford
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorREICHHARDT, Martin
dc.contributor.authorLUNDIN, Karolina
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorLOKKI, A
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorRECHER, Gaëlle
dc.contributor.authorVUORISTO, Sanna
hal.structure.identifierKarolinska Institutet [Stockholm]
dc.contributor.authorKATAYAMA, Shintaro
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorTAPANAINEN, Juha
hal.structure.identifierKarolinska Institutet [Stockholm]
hal.structure.identifierKing‘s College London
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorKERE, Juha
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
hal.structure.identifierHumanitas University [Milan] [Hunimed]
dc.contributor.authorMERI, Seppo
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorTUURI, Timo
dc.date.accessioned2023-05-12T10:44:25Z
dc.date.available2023-05-12T10:44:25Z
dc.date.issued2019-09-18
dc.identifier.issn1664-3224
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181700
dc.description.abstractEnIt is essential for early human life that mucosal immunological responses to developing embryos are tightly regulated. An imbalance of the complement system is a common feature of pregnancy complications. We hereby present the first full analysis of the expression and deposition of complement molecules in human pre-implantation embryos. Thus, far, immunological imbalance has been considered in stages of pregnancy following implantation. We here show that complement activation against developing human embryos takes place already at the pre-implantation stage. Using confocal microscopy, we observed deposition of activation products on healthy developing embryos, which highlights the need for strict complement regulation. We show that embryos express complement membrane inhibitors and bind soluble regulators. These findings show that mucosal complement targets human embryos, and indicate potential adverse pregnancy outcomes, if regulation of activation fails. In addition, single-cell RNA sequencing revealed cellular expression of complement activators. This shows that the embryonic cells themselves have the capacity to express and activate C3 and C5. The specific local embryonic expression of complement components, regulators, and deposition of activation products on the surface of embryos suggests that complement has immunoregulatory functions and furthermore may impact cellular homeostasis and differentiation at the earliest stages of life.
dc.language.isoen
dc.publisherFrontiers
dc.subject.enreproductive immunology
dc.subject.enmucosal immunology
dc.subject.encomplement
dc.subject.enembryo
dc.subject.endevelopment
dc.subject.enpre-implantation
dc.title.enComplement in Human Pre-implantation Embryos: Attack and Defense
dc.typeArticle de revue
dc.identifier.doi10.3389/fimmu.2019.02234
dc.subject.halSciences du Vivant [q-bio]/Biologie du développement
bordeaux.journalFrontiers in Immunology
bordeaux.volume1
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-02358138
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02358138v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Immunology&rft.date=2019-09-18&rft.volume=1&rft.eissn=1664-3224&rft.issn=1664-3224&rft.au=REICHHARDT,%20Martin&LUNDIN,%20Karolina&LOKKI,%20A&RECHER,%20Ga%C3%ABlle&VUORISTO,%20Sanna&rft.genre=article


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