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All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide

hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorALESSANDRI, Kévin
hal.structure.identifierLaboratoire Angiogenèse et Micro-environnement des Cancers [LAMC]
dc.contributor.authorANDRIQUE, Laëtitia
hal.structure.identifierInstitut des Maladies Neurodégénératives [Bordeaux] [IMN]
dc.contributor.authorFEYEUX, Maxime
hal.structure.identifierLaboratoire Angiogenèse et Micro-environnement des Cancers [LAMC]
dc.contributor.authorBIKFALVI, Andreas
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorRECHER, Gaëlle
dc.date.accessioned2023-05-12T10:38:15Z
dc.date.available2023-05-12T10:38:15Z
dc.date.issued2017-02-10
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181556
dc.description.abstractEnWhile live 3D high resolution microscopy techniques are developing rapidly, their use for biological applications is partially hampered by practical difficulties such as the lack of a versatile sample chamber. Here, we propose the design of a multi-usage observation chamber adapted for live 3D bio-imaging. We show the usefulness and practicality of this chamber, which we named the UniverSlide, for live imaging of two case examples, namely multicellular systems encapsulated in sub-millimeter hydrogel shells and zebrafish larvae. We also demonstrate its versatility and compatibility with all microscopy devices by using upright or inverted microscope configurations after loading the UniverSlide with fixed or living samples. Further, the device is applicable for medium/high throughput screening and automatized multi-position image acquisition, providing a constraint-free but stable and parallelized immobilization of the samples. The frame of the UniverSlide is fabricated using a stereolithography 3D printer, has the size of a microscopy slide, is autoclavable and sealed with a removable lid, which makes it suitable for use in a controlled culture environment. We describe in details how to build this chamber and we provide all the files necessary to print the different pieces in the lab.
dc.description.sponsorshipDéveloppment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
dc.description.sponsorshipTransgenèse pour les Etudes Fonctionnelles sur les Organismes modèles - ANR-11-INBS-0014
dc.description.sponsorshipLa tumeur et son environnement : Rôle du stress mécanique dans l'invasion des cellules cancéreuses - ANR-13-BSV5-0008
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enAll-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide
dc.typeArticle de revue
dc.identifier.doi10.1038/srep42378
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalScientific Reports
bordeaux.volume7
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01679318
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01679318v1
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