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A micromechanical cell stretching device compatible with super-resolution microscopy and single protein tracking

hal.structure.identifierInterdisciplinary Institute for Neuroscience [Bordeaux] [IINS]
dc.contributor.authorVICENTE, Filipe Nunes
hal.structure.identifierInterdisciplinary Institute for Neuroscience [Bordeaux] [IINS]
dc.contributor.authorMASSOU, Sophie
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorWETZEL, Franziska
hal.structure.identifierInterdisciplinary Institute for Neuroscience [Bordeaux] [IINS]
dc.contributor.authorMEHIDI, Amine
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorSTREHLE, Dan
hal.structure.identifierPolarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer
dc.contributor.authorLEDUC, Cecile
hal.structure.identifierLaboratoire Jean Perrin [LJP]
hal.structure.identifierLaboratoire de Physique Théorique de la Matière Condensée [LPTMC]
dc.contributor.authorVOITURIEZ, Raphaël
hal.structure.identifierInterdisciplinary Institute for Neuroscience [Bordeaux] [IINS]
dc.contributor.authorROSSIER, Olivier
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierInterdisciplinary Institute for Neuroscience [Bordeaux] [IINS]
dc.contributor.authorGIANNONE, Grégory
dc.date.accessioned2023-05-12T10:34:54Z
dc.date.available2023-05-12T10:34:54Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181482
dc.description.abstractEnCell mechano-sensing is based on biomolecule deformations and reorganizations, yet the molecular mechanisms are still unclear. Super-resolution microscopy (SRM) and single protein tracking (SPT) techniques reveal the dynamic organization of proteins at the nanoscale. In parallel, stretchable substrates are used to investigate cellular responses to mechanical forces. However, simultaneous combination of SRM/SPT and cell stretching has never been achieved. Here, we present a cell stretching device compatible with SRM and SPT, composed of an ultra-thin Polydimethylsiloxane (PDMS) layer. The PDMS sheet is gliding on a glycerol-lubricated glass cover-slip to ensure flatness during uniaxial stretching, generated with a 3D-printed micromechanical device by a mobile arm connected to a piezoelectric translator. This method enables to obtain super-resolved images of protein reorganization after live stretching, and to monitor single protein deformation and recruitment inside mechanosensitive structures upon stretching. This protocol is related to the publication ‘Cell stretching is amplified by active actin remodeling to deform and recruit proteins in mechanosensitive structures’, in Nature Cell Biology.
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.encytoskeleton
dc.subject.encell stretching
dc.subject.ensuperresolution microscopy
dc.subject.ensingle particle tracking
dc.subject.enmechanobiology
dc.subject.enmechanosensing
dc.subject.enfocal adhesions
dc.title.enA micromechanical cell stretching device compatible with super-resolution microscopy and single protein tracking
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.21203/rs.3.pex-961/v1
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
hal.identifierhal-03402139
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03402139v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=VICENTE,%20Filipe%20Nunes&MASSOU,%20Sophie&WETZEL,%20Franziska&MEHIDI,%20Amine&STREHLE,%20Dan&rft.genre=preprint


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