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Long-range transport of giant vesicles along microtubule networks

hal.structure.identifierBiotechnology Center [Dresden] [BIOTEC]
dc.contributor.authorHEROLD, Christophe
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLEDUC, Cecile
dc.contributor.authorROBERT, Stock
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorDIEZ, Stefan
hal.structure.identifierBiotechnology Center [Dresden] [BIOTEC]
dc.contributor.authorSCHWILLE, Petra
dc.date.accessioned2023-05-12T10:25:01Z
dc.date.available2023-05-12T10:25:01Z
dc.date.created2011-12-23
dc.date.issued2012-03-01
dc.identifier.issn1439-4235
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181234
dc.description.abstractEnWe report on a minimal system to mimic intracellular transport of membrane-bounded, vesicular cargo. In a cell-free assay, purified kinesin-1 motor proteins were directly anchored to the membrane of giant unilamellar vesicles, and their movement studied along two-dimensional microtubule networks. Motion-tracking of vesicles with diameters of 1-3 μm revealed traveling distances up to the millimeter range. The transport velocities were identical to velocities of cargo-free motors. Using total internal reflection fluorescence (TIRF) microscopy, we were able to estimate the number of GFP-labeled motors involved in the transport of a single vesicle. We found that the vesicles were transported by the cooperative activity of typically 5-10 motor molecules. The presented assay is expected to open up further applications in the field of synthetic biology, aiming at the in vitro reconstitution of sub-cellular multi-motor transport systems. It may also find applications in bionanotechnology, where the controlled long-range transport of artificial cargo is a promising means to advance current lab-on-a-chip systems.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subject.enmicrotubule
dc.subject.enbiomimetic system
dc.subject.envesicle transport
dc.subject.enkinesin
dc.title.enLong-range transport of giant vesicles along microtubule networks
dc.typeArticle de revue
dc.identifier.doi10.1002/cphc.201100669
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalChemPhysChem
bordeaux.page1001
bordeaux.volume13
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue4
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00759899
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00759899v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ChemPhysChem&rft.date=2012-03-01&rft.volume=13&rft.issue=4&rft.spage=1001&rft.epage=1001&rft.eissn=1439-4235&rft.issn=1439-4235&rft.au=HEROLD,%20Christophe&LEDUC,%20Cecile&ROBERT,%20Stock&DIEZ,%20Stefan&SCHWILLE,%20Petra&rft.genre=article


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