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High content Super-Resolution Imaging of Live Cell by uPAINT.

dc.contributor.authorGIANNONE, Gregory
dc.contributor.authorHOSY, Eric
dc.contributor.authorSIBARITA, Jean-Baptiste
dc.contributor.authorCHOQUET, Daniel
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorCOGNET, Laurent
dc.date.accessioned2023-05-12T10:24:39Z
dc.date.available2023-05-12T10:24:39Z
dc.date.issued2013
dc.identifier.issn1064-3745
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181221
dc.description.abstractEnIn this chapter, we present the uPAINT method (Universal Point Accumulation Imaging in Nanoscale Topography), a simple single-molecule super-resolution method which can be implemented on any wide field fluorescence microscope operating in oblique illumination. The key feature of uPAINT lies in recording high numbers of single molecules at the surface of a cell by constantly labeling while imaging. In addition to generating super-resolved images, uPAINT can provide dynamical information on a single live cell with large statistics revealing localization-specific diffusion properties of membrane biomolecules. Interestingly, any membrane biomolecule that can be labeled with a fluorescent ligand can be studied, making uPAINT an extremely versatile method.
dc.language.isoen
dc.publisherHumana Press/Springer Imprint
dc.title.enHigh content Super-Resolution Imaging of Live Cell by uPAINT.
dc.typeArticle de revue
dc.identifier.doi10.1007/978-1-62703-137-0_7
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalMethods in Molecular Biology
bordeaux.page95-110
bordeaux.volume950
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00796947
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00796947v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Methods%20in%20Molecular%20Biology&rft.date=2013&rft.volume=950&rft.spage=95-110&rft.epage=95-110&rft.eissn=1064-3745&rft.issn=1064-3745&rft.au=GIANNONE,%20Gregory&HOSY,%20Eric&SIBARITA,%20Jean-Baptiste&CHOQUET,%20Daniel&COGNET,%20Laurent&rft.genre=article


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