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Membrane tension regulates motility by controlling lamellipodium organization

hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorBATCHELDER, Ellen
hal.structure.identifierHoward Hughes Medical Institute, University of Utah
dc.contributor.authorHOLLOPETER, Gunther
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorCAMPILLO, Clément
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorMEZANGES, Xavier
hal.structure.identifierHoward Hughes Medical Institute, University of Utah
dc.contributor.authorJORGENSEN, Erik
hal.structure.identifierlp2n-04,lp2n-16
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratoire de Physico-Chimie Théorique [LPCT]
dc.contributor.authorSENS, Pierre
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorPLASTINO, Julie
dc.date.accessioned2023-05-12T10:23:48Z
dc.date.available2023-05-12T10:23:48Z
dc.date.created2010-07-19
dc.date.issued2011-07-12
dc.identifier.issn0027-8424
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181189
dc.description.abstractEnMany cell movements proceed via a crawling mechanism, where polymerization of the cytoskeletal protein actin pushes out the leading edge membrane. In this model, membrane tension has been seen as an impediment to filament growth and cell motility. Here we use a simple model of cell motility, the Caenorhabditis elegans sperm cell, to test how membrane tension affects movement and cytoskeleton dynamics. To enable these analyses, we create transgenic worm strains carrying sperm with a fluorescently labeled cytoskeleton. Via osmotic shock and deoxycholate treatments, we relax or tense the cell membrane and quantify apparent membrane tension changes by the membrane tether technique. Surprisingly, we find that membrane tension reduction is correlated with a decrease in cell displacement speed, whereas an increase in membrane tension enhances motility. We further demonstrate that apparent polymerization rates follow the same trends. We observe that membrane tension reduction leads to an unorganized, rough lamellipodium, composed of short filaments angled away from the direction of movement. On the other hand, an increase in tension reduces lateral membrane protrusions in the lamellipodium, and filaments are longer and more oriented toward the direction of movement. Overall we propose that membrane tension optimizes motility by streamlining polymerization in the direction of movement, thus adding a layer of complexity to our current understanding of how membrane tension enters into the motility equation.
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.title.enMembrane tension regulates motility by controlling lamellipodium organization
dc.typeArticle de revue
dc.identifier.doi10.1073/pnas.1010481108
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalProceedings of the National Academy of Sciences of the United States of America
bordeaux.page11429-11434
bordeaux.volume108
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue28
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00814820
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00814820v1
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