Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy
hal.structure.identifier | Laboratory for Bio- and Nano-Photonics, University of Freiburg | |
hal.structure.identifier | Max Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG] | |
dc.contributor.author | FAHRBACH, Florian | |
hal.structure.identifier | Physico-Chimie-Curie [PCC] | |
hal.structure.identifier | Compartimentation et dynamique cellulaires [CDC] | |
dc.contributor.author | GURCHENKOV, Vasily | |
hal.structure.identifier | Physico-Chimie-Curie [PCC] | |
hal.structure.identifier | Compartimentation et dynamique cellulaires [CDC] | |
dc.contributor.author | ALESSANDRI, Kévin | |
hal.structure.identifier | lp2n-04,lp2n-16 | |
hal.structure.identifier | Physico-Chimie-Curie [PCC] | |
dc.contributor.author | NASSOY, Pierre | |
hal.structure.identifier | Laboratory for Bio- and Nano-Photonics, University of Freiburg | |
hal.structure.identifier | Centre for Biological Signalling Studies (bioss) | |
dc.contributor.author | ROHRBACH, Alexander | |
dc.date.accessioned | 2023-05-12T10:22:19Z | |
dc.date.available | 2023-05-12T10:22:19Z | |
dc.date.created | 2013-02-12 | |
dc.date.issued | 2013-05-06 | |
dc.identifier.issn | 1094-4087 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181150 | |
dc.description.abstractEn | One of main challenges in light-sheet microscopy is to design the light-sheet as extended and thin as possible - extended to cover a large field of view, thin to optimize resolution and contrast. However, a decrease of the beam's waist also decreases the illumination beam's depth of field. Here, we introduce a new kind of beam that we call sectioned Bessel beam. These beams can be generated by blocking opposite sections of the beam's angular spectrum. In combination with confocal-line detection the optical sectioning performance of the light-sheet can be decoupled from the depth of field of the illumination beam. By simulations and experiments we demonstrate that these beams exhibit self-reconstruction capabilities and penetration depths into thick scattering media equal to those of conventional Bessel beams. We applied sectioned Bessel beams to illuminate tumor multicellular spheroids and prove the increase in contrast. Sectioned Bessel beams turn out to be highly advantageous for the investigation of large strongly scattering samples in a light-sheet microscope. | |
dc.language.iso | en | |
dc.publisher | Optical Society of America - OSA Publishing | |
dc.title.en | Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1364/OE.21.011425 | |
dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
dc.subject.hal | Sciences de l'ingénieur [physics]/Optique / photonique | |
bordeaux.journal | Optics Express | |
bordeaux.page | 11425-11440 | |
bordeaux.volume | 21 | |
bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
bordeaux.issue | 9 | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-00821366 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-00821366v1 | |
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