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Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy

hal.structure.identifierLaboratory for Bio- and Nano-Photonics, University of Freiburg
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorFAHRBACH, Florian
hal.structure.identifierPhysico-Chimie-Curie [PCC]
hal.structure.identifierCompartimentation et dynamique cellulaires [CDC]
dc.contributor.authorGURCHENKOV, Vasily
hal.structure.identifierPhysico-Chimie-Curie [PCC]
hal.structure.identifierCompartimentation et dynamique cellulaires [CDC]
dc.contributor.authorALESSANDRI, Kévin
hal.structure.identifierlp2n-04,lp2n-16
hal.structure.identifierPhysico-Chimie-Curie [PCC]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratory for Bio- and Nano-Photonics, University of Freiburg
hal.structure.identifierCentre for Biological Signalling Studies (bioss)
dc.contributor.authorROHRBACH, Alexander
dc.date.accessioned2023-05-12T10:22:19Z
dc.date.available2023-05-12T10:22:19Z
dc.date.created2013-02-12
dc.date.issued2013-05-06
dc.identifier.issn1094-4087
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181150
dc.description.abstractEnOne of main challenges in light-sheet microscopy is to design the light-sheet as extended and thin as possible - extended to cover a large field of view, thin to optimize resolution and contrast. However, a decrease of the beam's waist also decreases the illumination beam's depth of field. Here, we introduce a new kind of beam that we call sectioned Bessel beam. These beams can be generated by blocking opposite sections of the beam's angular spectrum. In combination with confocal-line detection the optical sectioning performance of the light-sheet can be decoupled from the depth of field of the illumination beam. By simulations and experiments we demonstrate that these beams exhibit self-reconstruction capabilities and penetration depths into thick scattering media equal to those of conventional Bessel beams. We applied sectioned Bessel beams to illuminate tumor multicellular spheroids and prove the increase in contrast. Sectioned Bessel beams turn out to be highly advantageous for the investigation of large strongly scattering samples in a light-sheet microscope.
dc.language.isoen
dc.publisherOptical Society of America - OSA Publishing
dc.title.enSelf-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy
dc.typeArticle de revue
dc.identifier.doi10.1364/OE.21.011425
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences de l'ingénieur [physics]/Optique / photonique
bordeaux.journalOptics Express
bordeaux.page11425-11440
bordeaux.volume21
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue9
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00821366
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00821366v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Optics%20Express&rft.date=2013-05-06&rft.volume=21&rft.issue=9&rft.spage=11425-11440&rft.epage=11425-11440&rft.eissn=1094-4087&rft.issn=1094-4087&rft.au=FAHRBACH,%20Florian&GURCHENKOV,%20Vasily&ALESSANDRI,%20K%C3%A9vin&NASSOY,%20Pierre&ROHRBACH,%20Alexander&rft.genre=article


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