Massive Parallelization of STED Nanoscopy Using Optical Lattices
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | YANG, Bin | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | PRZYBILLA, Frédéric | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | MESTRE, Michael | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | TREBBIA, Jean-Baptiste | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | LOUNIS, Brahim | |
| dc.date.accessioned | 2023-05-12T10:21:53Z | |
| dc.date.available | 2023-05-12T10:21:53Z | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181131 | |
| dc.description.abstractEn | Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-‐scanning. This constitutes a drawback for wide field imaging, since the gain in spatial resolution requires dense pixelation and hence long recording times. Here we achieve massive parallelization of STED nanoscopy using wide-‐field excitation together with well-‐designed optical lattices for depletion and a fast camera for detection. Acquisition of large field of view super-‐resolved images requires scanning over a single unit cell of the optical lattice which can be as small as 290 nm*290nm. Interference STED (In-‐STED) images of 2.9 μm* 2.9 μm with resolution down to 70 nm are obtained at 12.5 frames per second. The development of this technique opens many prospects for fast wide-‐field nanoscopy. | |
| dc.language.iso | en | |
| dc.title.en | Massive Parallelization of STED Nanoscopy Using Optical Lattices | |
| dc.type | Document de travail - Pré-publication | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Optique [physics.optics] | |
| dc.identifier.arxiv | 1307.3833 | |
| bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| hal.identifier | hal-00844210 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00844210v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=YANG,%20Bin&PRZYBILLA,%20Fr%C3%A9d%C3%A9ric&MESTRE,%20Michael&TREBBIA,%20Jean-Baptiste&LOUNIS,%20Brahim&rft.genre=preprint |
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