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Functional analysis and expression characteristics of chloroplastic Prx IIE

hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorGAMA, Filipe
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBRÉHÉLIN, Claire
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorGELHAYE, Éric
hal.structure.identifierLaboratoire Génome et développement des plantes [LGDP]
dc.contributor.authorMEYER, Yves
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorJACQUOT, Jean-Pierre
hal.structure.identifierAménagement, Développement, Environnement, Santé et Sociétés [ADES]
dc.contributor.authorREY, Pascal
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorROUHIER, Nicolas
dc.date.issued2008-07
dc.identifier.issn0031-9317
dc.description.abstractEnPeroxiredoxins (Prxs) are ubiquitous thiol-dependent peroxidases capable of eliminating a variety of peroxides through reactive catalytic cysteines, which are regenerated by reducing systems. Based on amino acid sequences and their mode of catalysis, five groups of thiol peroxidases have been distinguished in plants, and type II Prx is one of them with representatives in many sub-cellular compartments. The mature form of poplar chloroplastic Prx IIE was expressed as a recombinant protein in Escherichia coli. The protein is able to reduce H2O2 and tert-butyl hydroperoxide and is regenerated by both glutaredoxin (Grx) and thioredoxin (Trx) systems. Nevertheless, compared with Trxs, Grxs, and more especially chloroplastic Grx S12, are far more efficient reductants towards Prx IIE. The expression of Prx IIE at both the mRNA and protein levels as a function of organ type and abiotic stress conditions was investigated. Western blot analysis revealed that Prx IIE gene is constitutively expressed in Arabidopsis thaliana, mostly in young and mature leaves and in flowers. Under photo-oxidative treatment and water deficit, almost no change was observed in the abundance of Prx IIE in A. thaliana, while the level of Prx Q (one of the two other chloroplastic Prxs with 2-Cys Prx) increased in response to both stresses, indicating that plastidic members of the Prx family exhibit specific patterns of expression under stress.
dc.language.isoen
dc.publisherWiley
dc.subjectCHLOROPLAST GENETICS
dc.subjectFUNCTIONAL GENOMICS
dc.subjectPLANTS
dc.subject.enGENE EXPRESSION
dc.subject.enGENES
dc.subject.enARABIDOPSIS THALIANA
dc.title.enFunctional analysis and expression characteristics of chloroplastic Prx IIE
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1399-3054.2008.01097.x
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPhysiologia Plantarum
bordeaux.page599-610
bordeaux.volume133
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02118623
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02118623v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Physiologia%20Plantarum&rft.date=2008-07&rft.volume=133&rft.issue=3&rft.spage=599-610&rft.epage=599-610&rft.eissn=0031-9317&rft.issn=0031-9317&rft.au=GAMA,%20Filipe&BR%C3%89H%C3%89LIN,%20Claire&GELHAYE,%20%C3%89ric&MEYER,%20Yves&JACQUOT,%20Jean-Pierre&rft.genre=article


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