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Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP- l -galactose phosphorylase

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOURNONVILLE, Céline
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDESLOUS, Paul
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROS, Guillaume
hal.structure.identifierHeinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf]
dc.contributor.authorBLOMEIER, Tim
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGADIN, Stéphanie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCASSAN, Cédric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickael
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJUST, Daniel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRÈS, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERNANDEZ-LOCHU, Lucie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATAILLE, Laure
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorMIURA, Kenji
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierHeinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf]
dc.contributor.authorZURBRIGGEN, Matias
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPÉTRIACQ, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
dc.date.issued2023-04-13
dc.identifier.issn1040-4651
dc.description.abstractEnAbstract Ascorbate (vitamin C) is an essential antioxidant in fresh fruits and vegetables. To gain insight into the regulation of ascorbate metabolism in plants, we studied mutant tomato plants (Solanum lycopersicum) that produce ascorbate-enriched fruits. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to a knock-out recessive mutation in a class of photoreceptor named PAS/LOV protein (PLP), which acts as a negative regulator of ascorbate biosynthesis. This trait was confirmed by CRISPR/Cas9 gene editing and further found in all plant organs, including fruit that accumulated 2 to 3 times more ascorbate than in the WT. The functional characterization revealed that PLP interacted with the 2 isoforms of GDP-l-galactose phosphorylase (GGP), known as the controlling step of the l-galactose pathway of ascorbate synthesis. The interaction with GGP occurred in the cytoplasm and the nucleus, but was abolished when PLP was truncated. These results were confirmed by a synthetic approach using an animal cell system, which additionally demonstrated that blue light modulated the PLP-GGP interaction. Assays performed in vitro with heterologously expressed GGP and PLP showed that PLP is a noncompetitive inhibitor of GGP that is inactivated after blue light exposure. This discovery provides a greater understanding of the light-dependent regulation of ascorbate metabolism in plants.
dc.description.sponsorshipCentre français de phénomique végétale
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subjectTomate
dc.subjectSolanum Lycopersicum
dc.subjectAscorbate
dc.subjectvitamine c
dc.subjectmétabolisme de l'ascorbate
dc.subjectLumière
dc.title.enBlue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP- l -galactose phosphorylase
dc.typeArticle de revue
dc.identifier.doi10.1093/plcell/koad108
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalThe Plant cell
bordeaux.page2615-2634
bordeaux.volume35
bordeaux.issue7
bordeaux.peerReviewedoui
hal.identifierhal-04083263
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04083263v1
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