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Mis-expression of a PISTILLATA-like MADS box gene prevents fruit development in grapevine

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierInstituto de Ciencias de la Vid y del Vino (Consejo Superior de Investigaciones Cientıficas)
dc.contributor.authorFERNANDEZ, Lucie
hal.structure.identifierCommonwealth Scientific and Industrial Research Organisation [Australia] [CSIRO]
dc.contributor.authorCHAÏB, Jamila
hal.structure.identifierInstituto de Ciencias de la Vid y del Vino (Consejo Superior de Investigaciones Cientıficas)
dc.contributor.authorMARTINEZ-ZAPATER, José-Miguel
hal.structure.identifierPlant Industry
dc.contributor.authorTHOMAS, Mark R
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorTORREGROSA, Laurent
dc.date.issued2013
dc.identifier.issn0960-7412
dc.description.abstractEnThe FLESHLESS BERRY (Flb) somatic variant identified in the grapevine cultivar Ugni Blanc develops grape berries without flesh, suggesting a role for the altered gene in differentiation of flesh cells. Here we describe identification of the molecular defect responsible for this phenotype. Using a combination of genetic and transcriptomic approaches, we detected the insertion of a miniature inverted-repeat transposable element in the promoter region of the PISTILLATA-like (VvPI) gene, the grapevine homologue of Arabidopsis PISTILLATA. The transposon insertion causes specific ectopic expression of the corresponding VvPI allele during early fruit development, causing expression of genes specific for petal and stamen development within the fruit. A causal relationship between the insertion and the phenotype was demonstrated by phenotypic and molecular analyses of somatic revertants showing that ectopic expression and mutant phenotype were always linked to the presence of the transposon insertion. The various phenotypic effects of the flb mutation on ovary morphology, fruit set and fruit development, depending on the cell lineage affected, are presented for each phenotype, offering new insights into floral and fleshly fruit development. The results highlight the importance of VvPI repression after fertilization to achieve normal fleshy fruit development, and the complex genetic, genomic and cellular interactions required for the flower to fruit transition in grapevine.
dc.language.isoen
dc.publisherWiley
dc.subject.enmorphogenesis
dc.subject.enfruit
dc.subject.enflesh
dc.subject.enPISTILLATA
dc.subject.enactive transposition
dc.subject.ensomatic variation
dc.subject.enVitis vinifera
dc.title.enMis-expression of a PISTILLATA-like MADS box gene prevents fruit development in grapevine
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.12083
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Alimentation et Nutrition
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie du développement
bordeaux.journalThe Plant Journal
bordeaux.page918-28
bordeaux.volume73
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-01506200
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01506200v1
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