Afficher la notice abrégée

Probing the threshold of membrane damage and cytotoxicity effects induced by silica nanoparticles in Escherichia coli bacteria

hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMATHELIÉ-GUINLET, Marion
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉVEN, Laure
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMOROTÉ, Fabien
hal.structure.identifierBiothérapies des maladies génétiques et cancers
dc.contributor.authorMOYNET, Daniel
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
hal.structure.identifierCellule de transfert ADERA NanoPhyNov
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGAMMOUDI, Ibtissem
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
dc.date.issued2017
dc.identifier.issn0001-8686
dc.description.abstractEnThe engineering of nanomaterials, because of their specific properties, is increasingly being developed for commercial purposes over the past decades, to enhance diagnosis, cosmetics properties as well as sensing efficiency. However, the understanding of their fate and thus their interactions at the cellular level with bio-organisms remains elusive. Here, we investigate the size-and charge-dependence of the damages induced by silica nanoparticles (SiO 2-NPs) on Gram-negative Escherichia coli bacteria. We show and quantify the existence of a NPs size threshold discriminating toxic and inert SiO 2-NPs with a critical particle diameter (Φ c) in the range 50nm–80nm. This particular threshold is identified at both the micrometer scale via viability tests through Colony Forming Units (CFU) counting, and the nanometer scale via atomic force microscopy (AFM). At this nanometer scale, AFM emphasizes the interaction between the cell membrane and SiO 2-NPs from both topographic and mechanical points of view. For SiO 2-NPs with Φ > Φ c no change in E. coli morphology nor its outer membrane (OM) organization is observed unless the NPs are positively charged in which case reorganization and disruption of the OM are detected. Conversely, when Φ < Φ c , E. coli exhibit unusual spherical shapes, partial collapse, even lysis, and OM reorganization.
dc.language.isoen
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subject.enSilica nanoparticles
dc.subject.enMembrane
dc.subject.enToxicity
dc.subject.enAFM
dc.subject.enE coli
dc.title.enProbing the threshold of membrane damage and cytotoxicity effects induced by silica nanoparticles in Escherichia coli bacteria
dc.typeArticle de revue
dc.identifier.doi10.1016/j.cis.2017.04.012
dc.subject.halChimie/Matériaux
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie
bordeaux.journalAdvances in Colloid and Interface Science
bordeaux.page81-91
bordeaux.volume245
bordeaux.peerReviewedoui
hal.identifierhal-01540158
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01540158v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advances%20in%20Colloid%20and%20Interface%20Science&rft.date=2017&rft.volume=245&rft.spage=81-91&rft.epage=81-91&rft.eissn=0001-8686&rft.issn=0001-8686&rft.au=MATHELI%C3%89-GUINLET,%20Marion&B%C3%89VEN,%20Laure&MOROT%C3%89,%20Fabien&MOYNET,%20Daniel&GRAUBY-HEYWANG,%20Christine&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée