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A multi-Ievel omic approach of tomato fruit quality

hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
hal.structure.identifierCollege of horticulture
dc.contributor.authorXU, Jiaxin
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorPASCUAL-BANULS, Laura
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorDESPLAT, Nelly
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
hal.structure.identifierCollege of horticulture
dc.contributor.authorFAUROBERT, Mireille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickaël
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALLIAS, P.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorBOUCHET, Jean-Paul
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorBRUNEL, Dominique
hal.structure.identifierUR1279, Unité Etude du Polymorphisme des Génomes Végétaux, CEA-Institut de Génomique-CNG
dc.contributor.authorLE PASLIER, Marie-Christine
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorCAUSSE, Mathilde
dc.date.issued2015
dc.identifier.issn0567-7572
dc.description.abstractEnImprovement of fruit quality traits is a major goal for tomato breeding. Deciphering the genetic diversity and inheritance of fruit quality components is thus necessary. For this purpose, we carried out a large multi-level omic experiment. Eight contrasted lines and 4 of their F-1 hybrids were phenotyped for fruit development traits. Fruit pericarp samples were analysed at 2 stages (cell expansion and orange ripe) and different scales: (1) untargeted profiling of major polar metabolites, (2) activities of 28 enzymes involved in primary metabolism, (3) proteome profiles revealed by 2D-PAGE and identification of 470 protein spots showing quantitative variations and (4) gene expression analysis by Digital Gene Expression. In parallel, the 8 lines were resequenced and more than 3 million SNPs identified when aligned on the reference tomato genome. This experiment allowed us to assess and compare the range of variability and inheritance mode of the metabolic traits and expression data. Correlation networks were constructed within and between levels of analysis to identify regulatory networks. Diversity of candidate genes could thus be analysed, relating the polymorphisms at the sequence levels with their expression.
dc.language.isoen
dc.publisherInternational Society for Horticultural Science
dc.subjectqualité du fruit
dc.subjectcaractère polygénique
dc.subjectdiversité génétique
dc.subjectdéveloppement du fruit
dc.subjectexpression des gènes
dc.subjectactivité enzymatique
dc.subjecttomate
dc.subjectsolanum lycopersicum
dc.subjectprotéome
dc.subjecttranscriptome
dc.subjectmétabolome
dc.subjectcellule de péricarpe
dc.subjectapproche multi-omique
dc.subjectbiologie des systèmes
dc.subjectprofil métabolique
dc.subjectséquençage de gènes
dc.subjectprofil protéomique
dc.subjectpolymorphisme snp
dc.subject.enfruit quality
dc.subject.engenomic
dc.subject.enSNP
dc.subject.engenêtic variation
dc.subject.enenzymic activity
dc.subject.entomato
dc.subject.ensystems biology
dc.title.enA multi-Ievel omic approach of tomato fruit quality
dc.typeArticle de revue
dc.identifier.doi10.17660/ActaHortic.2015.1099.100
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalActa Horticulturae
bordeaux.page793-800
bordeaux.volume1099
bordeaux.peerReviewedoui
hal.identifierhal-01543456
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01543456v1
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