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Ozone affects ascorbate and glutathione biosynthesis as well as amino acid contents in three Euramerican poplar genotypes

hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
hal.structure.identifierUniversity of Eastern Finland
dc.contributor.authorDUMONT, J.
hal.structure.identifierUniversity of Eastern Finland
dc.contributor.authorKESKI-SAARI, S.
hal.structure.identifierUniversity of Eastern Finland
dc.contributor.authorKEINANEN, M.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorCOHEN, D.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorNINGRE, N.
hal.structure.identifierUniversity of Eastern Finland
dc.contributor.authorKONTUNEN-SOPPELA, S.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorBALDET, P.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorGIBON, Y.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorDIZENGREMEL, P.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorVAULTIER, M.-N.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorJOLIVET, Y.
hal.structure.identifierUniversity of Eastern Finland
dc.contributor.authorOKSANEN, E.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
hal.structure.identifierEcosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation [EFABA]
dc.contributor.authorLE THIEC, D.
dc.date.issued2014
dc.identifier.issn0829-318X
dc.description.abstractEnOzone is an air pollutant that causes oxidative stress by generation of reactive oxygen species (ROS) within the leaf. The capacity to detoxify ROS and repair ROS-induced damage may contribute to ozone tolerance. Ascorbate and glutathione are known to be key players in detoxification. Ozone effects on their biosynthesis and on amino acid metabolism were investigated in three Euramerican poplar genotypes (Populus deltoides Bartr. x Populus nigra L.) differing in ozone sensitivity. Total ascorbate and glutathione contents were increased in response to ozone in all genotypes, with the most resistant genotype (Carpaccio) showing an increase of up to 70%. Reduced ascorbate (ASA) concentration at least doubled in the two most resistant genotypes (Carpaccio and Cima), whereas the most sensitive genotype (Robusta) seemed unable to regenerate ASA from oxidized ascorbate (DHA), leading to an increase of 80% of the oxidized form. Increased ascorbate (ASA + DHA) content correlated with the increase in gene expression in its biosynthetic pathway, especially the putative gene of GDP-L-galactose phosphorylase VTC2. Increased cysteine availability combined with increased expression of gamma-glutamylcysteine synthetase (GSH1) and glutathione synthetase (GSH2) genes allows higher glutathione biosynthesis in response to ozone, particularly in Carpaccio. In addition, ozone caused a remobilization of amino acids with a decreased pool of total amino acids and an increase of Cys and putrescine, especially in Carpaccio. In addition, the expression of genes encoding threonine aldolase was strongly induced only in the most tolerant genotype, Carpaccio. Reduced ascorbate levels could partly explain the sensitivity to ozone for Robusta but not for Cima. Reduced ascorbate level alone is not sufficient to account for ozone tolerance in poplar, and it is necessary to consider several other factors including glutathione content.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.enair pollution
dc.subject.enantioxidants
dc.subject.enmetabolism
dc.subject.enoxidative stress
dc.subject.enPopulus
dc.title.enOzone affects ascorbate and glutathione biosynthesis as well as amino acid contents in three Euramerican poplar genotypes
dc.typeArticle de revue
dc.identifier.doi10.1093/treephys/tpu004
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalTree Physiology
bordeaux.page253 - 266
bordeaux.volume34
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-01555992
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01555992v1
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