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Non-structural carbohydrates in woody plants compared among laboratories

hal.structure.identifierCommonwealth Scientific and Industrial Research Organisation [Australia] [CSIRO]
hal.structure.identifierWestern Sydney University
dc.contributor.authorQUENTIN, Audrey G.
hal.structure.identifierCommonwealth Scientific and Industrial Research Organisation [Australia] [CSIRO]
dc.contributor.authorPINKARD, Elizabeth A.
hal.structure.identifierColorado State University [Fort Collins] [CSU]
hal.structure.identifierUnited States Department of Agriculture [USDA]
dc.contributor.authorRYAN, Michael G.
hal.structure.identifierWestern Sydney University
dc.contributor.authorTISSUE, David T.
hal.structure.identifierUnited States Department of Agriculture [USDA]
dc.contributor.authorBAGGETT, L. Scott
hal.structure.identifierLos Alamos National Laboratory [LANL]
dc.contributor.authorADAMS, Henry D.
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
dc.contributor.authorMAILLARD, Pascale
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
dc.contributor.authorMARCHAND, Marc
hal.structure.identifierUniversity of Alberta
dc.contributor.authorLANDHÄUSSER, Simon M.
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
dc.contributor.authorLACOINTE, André
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorGIBON, Yves
hal.structure.identifierPrinceton University
dc.contributor.authorANDEREGG, William R.L.
hal.structure.identifierColorado State University [Fort Collins] [CSU]
dc.contributor.authorASAO, Shinichi
hal.structure.identifierAustralian National University [ANU]
dc.contributor.authorATKIN, Owen K.
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
dc.contributor.authorBONHOMME, Marc
hal.structure.identifierUniversity of Tasmania [Hobart] [UTAS]
dc.contributor.authorCLAYE, Caroline
hal.structure.identifierUniversity of Alberta
dc.contributor.authorCHOW, Pak S.
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorCLÉMENT-VIDAL, Anne
hal.structure.identifierUniversity of Tasmania [Hobart] [UTAS]
dc.contributor.authorDAVIES, Noel W.
hal.structure.identifierLos Alamos National Laboratory [LANL]
dc.contributor.authorDICKMAN, L. Turin
hal.structure.identifierThe Hebrew University of Jerusalem [HUJ]
dc.contributor.authorDUMBUR, Rita
hal.structure.identifierWestern Sydney University
dc.contributor.authorELLSWORTH, David S.
hal.structure.identifierOregon State University [OSU]
dc.contributor.authorFALK, Kristen
hal.structure.identifierSwiss Federal Institute for Forest, Snow and Landscape Research WSL
hal.structure.identifierAlbert-Ludwigs-Universität Freiburg = University of Freiburg
dc.contributor.authorGALIANO, Lucía
hal.structure.identifierThe Hebrew University of Jerusalem [HUJ]
dc.contributor.authorGRÜNZWEIG, José M.
hal.structure.identifierMax-Planck-Institut für Biogeochemie [MPI-BGC]
dc.contributor.authorHARTMANN, Henrik
hal.structure.identifierUniversité de Bâle = University of Basel = Basel Universität [Unibas]
dc.contributor.authorHOCH, Günter
hal.structure.identifierMontana State University [MSU]
dc.contributor.authorHOOD, Sharon
hal.structure.identifierUniversity of Tasmania [Hobart] [UTAS]
dc.contributor.authorJONES, Joanna E.
hal.structure.identifierHokkaido University [Sapporo, Japan]
dc.contributor.authorKOIKE, Takayoshi
hal.structure.identifierMax-Planck-Institut für Biogeochemie [MPI-BGC]
dc.contributor.authorKUHLMANN, Iris
hal.structure.identifierUniversitat de Barcelona [UB]
dc.contributor.authorLLORET, Francisco
hal.structure.identifierInstituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] [IPE - CSIC]
dc.contributor.authorMAESTRO, Melchor
hal.structure.identifierUniversity of British Columbia [Canada] [UBC]
dc.contributor.authorMANSFIELD, Shawn D.
hal.structure.identifierUniversitat de Barcelona [UB]
dc.contributor.authorMARTÍNEZ-VILALTA, Jordi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorMAUCOURT, Mickael
hal.structure.identifierLos Alamos National Laboratory [LANL]
dc.contributor.authorMCDOWELL, Nathan G.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierÉcophysiologie des Plantes sous Stress environnementaux [LEPSE]
dc.contributor.authorMULLER, Bertrand
hal.structure.identifierUniversitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia [UPV]
dc.contributor.authorNEBAUER, Sergio G.
hal.structure.identifierInstituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] [IPE - CSIC]
dc.contributor.authorPALACIO, Sara
hal.structure.identifierCentro de Investigación en Ecosistemas de la Patagonia - Universidad Austral de Chile [CIEP]
dc.contributor.authorPIPER, Frida
dc.contributor.authorRAVEH, Eran
hal.structure.identifierUniversität Wien = University of Vienna
dc.contributor.authorRICHTER, Andreas
hal.structure.identifierÉcophysiologie des Plantes sous Stress environnementaux [LEPSE]
dc.contributor.authorROLLAND, Gaëlle
hal.structure.identifierUniversitat de Barcelona [UB]
dc.contributor.authorROSAS, Teresa
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
dc.contributor.authorSAINT JOANIS, Brigitte
hal.structure.identifierMontana State University [MSU]
dc.contributor.authorSALA, Anna
dc.contributor.authorSMITH, Renee A.
hal.structure.identifierWageningen University and Research [Wageningen] [WUR]
dc.contributor.authorSTERCK, Frank
hal.structure.identifierUniversity of Western Ontario [UWO]
dc.contributor.authorSTINZIANO, Joseph R.
hal.structure.identifierEstonian University of Life Sciences [EMU]
dc.contributor.authorTOBIAS, Mari
hal.structure.identifierUniversity of British Columbia [Canada] [UBC]
dc.contributor.authorUNDA, Faride
hal.structure.identifierTokyo University of Agriculture and Technology [TUAT]
dc.contributor.authorWATANABE, Makoto
hal.structure.identifierDuke University [Durham]
hal.structure.identifierUniversity of Western Ontario [UWO]
dc.contributor.authorWAY, Danielle A.
hal.structure.identifierUniversity of Peradeniya
hal.structure.identifierAustralian National University [ANU]
dc.contributor.authorWEERASINGHE, Lasantha K.
hal.structure.identifierGöteborgs Universitet = University of Gothenburg [GU]
hal.structure.identifierUniversität Wien = University of Vienna
dc.contributor.authorWILD, Birgit
hal.structure.identifierUniversity of Alberta
dc.contributor.authorWILEY, Erin
hal.structure.identifierUSDA Agricultural Research Service [Maricopa, AZ] [USDA]
dc.contributor.authorWOODRUFF, David R.
dc.date.issued2015
dc.identifier.issn0829-318X
dc.description.abstractEnNon-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g(-1) for soluble sugars, 6-533 (mean = 94) mg g-1 for starch and 53-649 (mean = 153) mg g-1 for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R-2 = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g-1 for total NSC, compared with the range of laboratory estimates of 596 mg g-1. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectExtraction and quantification consistency
dc.subject.enNon-structural carbohydrate chemical analysis
dc.subject.enParticle size
dc.subject.enReference method
dc.subject.enSoluble sugars
dc.subject.enStandardization
dc.subject.enStarch
dc.title.enNon-structural carbohydrates in woody plants compared among laboratories
dc.typeArticle de revue
dc.identifier.doi10.1093/treephys/tpv073
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalTree Physiology
bordeaux.page1146-1165
bordeaux.volume35
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-01557364
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01557364v1
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