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Specific evolution of F1-like ATPases in mycoplasmas.

hal.structure.identifierGénie Enzymatique et Cellulaire [GEC]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉVEN, Laure
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCHARENTON, Claire
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorDAUTANT, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOUYSSOU, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLABROUSSAA, Fabien
dc.contributor.authorSKÖLLERMO, Anna
dc.contributor.authorPERSSON, Anja
hal.structure.identifierGénomique, développement et pouvoir pathogène [GD2P]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierGénomique, développement et pouvoir pathogène [GD2P]
dc.contributor.authorSIRAND-PUGNET, Pascal
dc.date.issued2012
dc.identifier.issn1932-6203
dc.description.abstractEnF(1)F(0) ATPases have been identified in most bacteria, including mycoplasmas which have very small genomes associated with a host-dependent lifestyle. In addition to the typical operon of eight genes encoding genuine F(1)F(0) ATPase (Type 1), we identified related clusters of seven genes in many mycoplasma species. Four of the encoded proteins have predicted structures similar to the α, β, γ and ε subunits of F(1) ATPases and could form an F(1)-like ATPase. The other three proteins display no similarity to any other known proteins. Two of these proteins are probably located in the membrane, as they have three and twelve predicted transmembrane helices. Phylogenomic studies identified two types of F(1)-like ATPase clusters, Type 2 and Type 3, characterized by a rapid evolution of sequences with the conservation of structural features. Clusters encoding Type 2 and Type 3 ATPases were assumed to originate from the Hominis group of mycoplasmas. We suggest that Type 3 ATPase clusters may spread to other phylogenetic groups by horizontal gene transfer between mycoplasmas in the same host, based on phylogeny and genomic context. Functional analyses in the ruminant pathogen Mycoplasma mycoides subsp. mycoides showed that the Type 3 cluster genes were organized into an operon. Proteomic analyses demonstrated that the seven encoded proteins were produced during growth in axenic media. Mutagenesis and complementation studies demonstrated an association of the Type 3 cluster with a major ATPase activity of membrane fractions. Thus, despite their tendency toward genome reduction, mycoplasmas have evolved and exchanged specific F(1)-like ATPases with no known equivalent in other bacteria. We propose a model, in which the F(1)-like structure is associated with a hypothetical X(0) sector located in the membrane of mycoplasma cells.
dc.language.isoen
dc.publisherPublic Library of Science
dc.subjectruminant
dc.subjectbactérie pathogène
dc.subjectmollicute
dc.subjectphylogénomique
dc.subjectmycoplasma mycoides
dc.subjectgénomique comparée
dc.subjectmycoplasme
dc.subjectatpase
dc.subjectpathologie animale
dc.subjectsanté animale
dc.subjectphylogénétique
dc.subject.engenome
dc.subject.enanimal pathology
dc.subject.enmycoplasma
dc.subject.enanimal health
dc.subject.enpathogenic bacteria
dc.title.enSpecific evolution of F1-like ATPases in mycoplasmas.
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0038793
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
bordeaux.journalPLoS ONE
bordeaux.pagee38793
bordeaux.volume7
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-00735323
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00735323v1
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