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Characterization of a NADH-dependent glutamate dehydrogenase mutant of [i]Arabidopsis[/i] demonstrates the key role of this enzyme in root carbon and nitrogen metabolism

hal.structure.identifierBiologie des Plantes et Innovation - UR UPJV 3900 [BIOPI]
dc.contributor.authorFONTAINE, Jean-Xavier
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorTERCÉ-LAFORGUE, Therese
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorARMENGAUD, Patrick
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorCLÉMENT, Gilles
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorRENOU, Jean-Pierre
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorPELLETIER, Sandra
hal.structure.identifierEcologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 [EDYSAN]
dc.contributor.authorCATTEROU, Manuella
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorAZZOPARDI, Marianne
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierRothamsted Research
dc.contributor.authorLEA, Peter J.
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorHIREL, Bertrand
hal.structure.identifierEcologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 [EDYSAN]
dc.contributor.authorDUBOIS, Frédéric
dc.date.issued2012-10
dc.identifier.issn1040-4651
dc.description.abstractEnThe role of NADH-dependent glutamate dehydrogenase (GDH) was investigated by studying the physiological impact of a complete lack of enzyme activity in an Arabidopsis thaliana plant deficient in three genes encoding the enzyme. This study was conducted following the discovery that a third GDH gene is expressed in the mitochondria of the root companion cells, where all three active GDH enzyme proteins were shown to be present. A gdh1-2-3 triple mutant was constructed and exhibited major differences from the wild type in gene transcription and metabolite concentrations, and these differences appeared to originate in the roots. By placing the gdh triple mutant under continuous darkness for several days and comparing it to the wild type, the evidence strongly suggested that the main physiological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle. The differences in key metabolites of the tricarboxylic acid cycle in the triple mutant versus the wild type indicated that, through metabolic processes operating mainly in roots, there was a strong impact on amino acid accumulation, in particular alanine, gamma-aminobutyrate, and aspartate in both roots and leaves. These results are discussed in relation to the possible signaling and physiological functions of the enzyme at the interface of carbon and nitrogen metabolism.
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.title.enCharacterization of a NADH-dependent glutamate dehydrogenase mutant of [i]Arabidopsis[/i] demonstrates the key role of this enzyme in root carbon and nitrogen metabolism
dc.typeArticle de revue
dc.identifier.doi10.1105/tpc.112.103689
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalThe Plant cell
bordeaux.page4044-4065
bordeaux.volume24
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-01019522
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01019522v1
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