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Random transposon insertion in the<em> Mycoplasma hominis</em> minimal genome.

hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorRIDEAU, Fabien
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorLE ROY, Chloé
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorSAGNÉ, Evelyne
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorRENAUDIN, Hélène
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorPEREYRE, Sabine
hal.structure.identifierHeinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf]
hal.structure.identifierInstitute of Medical Microbiology and Hospital Hygiene [University of Düsseldorf]
dc.contributor.authorHENRICH, Birgit
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDORDET-FRISONI, Emilie
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorBEBEAR, Cécile
dc.date.issued2019-09-19
dc.identifier.issn2045-2322
dc.description.abstractEnMycoplasma hominis is an opportunistic human pathogen associated with genital and neonatal infections. Until this study, the lack of a reliable transformation method for the genetic manipulation of M. hominis hindered the investigation of the pathogenicity and the peculiar arginine-based metabolism of this bacterium. A genomic analysis of 20 different M. hominis strains revealed a number of putative restriction-modification systems in this species. Despite the presence of these systems, a reproducible polyethylene glycol (PEG)-mediated transformation protocol was successfully developed in this study for three different strains: two clinical isolates and the M132 reference strain. Transformants were generated by transposon mutagenesis with an efficiency of approximately 10−9 transformants/cell/µg plasmid and were shown to carry single or multiple mini-transposons randomly inserted within their genomes. One M132-mutant was observed to carry a single-copy transposon inserted within the gene encoding P75, a protein potentially involved in adhesion. However, no difference in adhesion was observed in cell-assays between this mutant and the M132 parent strain. Whole genome sequencing of mutants carrying multiple copies of the transposon further revealed the occurrence of genomic rearrangements. Overall, this is the first time that genetically modified strains of M. hominis have been obtained by random mutagenesis using a mini-transposon conferring resistance to tetracycline.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enRandom transposon insertion in the<em> Mycoplasma hominis</em> minimal genome.
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-019-49919-y
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie
bordeaux.journalScientific Reports
bordeaux.page13554
bordeaux.volume9
bordeaux.peerReviewedoui
hal.identifierhal-02618351
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02618351v1
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