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Genome characterization of a divergent isolate of the mycovirus Botrytis virus F from a grapevine metagenome.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSVANELLA-DUMAS, Laurence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTHEIL, Sébastien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEFEBVRE, Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2018
dc.identifier.issn0304-8608
dc.description.abstractEnAs part of a grapevine metagenome study, total RNA extracted from grapevine phloem scrapings was analyzed by Illumina sequencing. For one 420A rootstock sample, reads mapping against a reference database and BLAST annotation of contigs identified the presence of a divergent isolate of Botrytis virus F (BVF). The full genome sequence of this isolate (IVC-5-77) was determined (6,828 nucleotides [nt], excluding the poly(A) tail) and shown to be collinear with that of the BVF reference isolate, with the two open reading frames encoding a replication-associated protein (REP) and a coat protein (CP). The IVC-5-77 isolate, however, is very divergent, showing only 81.3-81.6% nucleotide sequence identity to the two other sequenced BVF isolates. The internal non-coding region was also found to be highly variable between BVF isolates. Analysis of the RNASeq reads demonstrated that close to 20% of them belong to Botrytis cinerea, the putative host of the IVC-5-77 isolate. These results extend our knowledge of the diversity and variability of BVF and demonstrate its detectability, together with that of its B. cinerea host, in total RNA RNASeq data from grapevine phloem scrapings.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subject.enBotrytis virus F
dc.title.enGenome characterization of a divergent isolate of the mycovirus Botrytis virus F from a grapevine metagenome.
dc.typeArticle de revue
dc.identifier.doi10.1007/s00705-018-3975-7
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalArchives of Virology
bordeaux.page3181-3183
bordeaux.volume163
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-02621857
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02621857v1
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