High-Throughput Sequencing Reveals <em>Cyclamen persicum</em> Mill. as a Natural Host for Fig Mosaic Virus.
| hal.structure.identifier | Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Bari [CIHEAM-IAMB] | |
| dc.contributor.author | ELBEAINO, Toufic | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | MARAIS, Armelle | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | FAURE, Chantal | |
| hal.structure.identifier | National Research Council of Italy | Consiglio Nazionale delle Ricerche [CNR] | |
| dc.contributor.author | TRIOANO, Elisa | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | CANDRESSE, Thierry | |
| hal.structure.identifier | National Research Council of Italy | Consiglio Nazionale delle Ricerche [CNR] | |
| dc.contributor.author | PARRELLA, Giuseppe | |
| dc.date.issued | 2018 | |
| dc.identifier.issn | 1999-4915 | |
| dc.description.abstractEn | In a search for viral infections, double-stranded RNA (dsRNA) were recovered from a diseased cyclamen (<em>Cyclamen persicum</em> Mill.) accession (Cic) and analyzed by high-throughput sequencing (HTS) technology. Analysis of the HTS data showed the presence of Fig mosaic emaravirus (FMV) in this accession. The complete sequences of six FMV-Cic RNA genomic segments were determined from the HTS data and using Sanger sequencing. All FMV-Cic RNA segments are similar in size to those of FMV from fig (FMV-Gr10), with the exception of RNA-6 that is one nucleotide longer. The occurrence of FMV in cyclamen was investigated through a small-scale survey, from which four plants (out of 18 tested) were found RT-PCR positive. To study sequence variations of cyclamen isolates of FMV, RT-PCR products generated through the amplification of the partially RNA-dependent RNA polymerase (RdRp, RNA-1), glycoprotein (GP, RNA-2), and nucleocapsid (NCP, RNA-3) genes were explored. The nucleotide sequence identities for cyclamen isolates ranged between 86% and 99% in RNA-1, 93% and 99% in RNA-2, and 98% and 99% in RNA-3, while lower identity levels were observed with the sequences of fig isolates. Based on the phylogenetic tree obtained with a 304-nt fragment of RNA3, all FMV isolates from cyclamens were assigned to a single cluster close to fig isolates from the Mediterranean. FMV was graft-transmitted to healthy cyclamens eliciting symptoms similar to those observed in the Cic accession, thus suggesting a causal role of FMV in the symptoms that prompted the investigation. This is the first report of FMV in a non-fig host, <em>Cyclamen persicum</em>, a finding that may help in the control of the mosaic and mosaic-like diseases of fig and cyclamen, respectively. | |
| dc.language.iso | en | |
| dc.publisher | MDPI | |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject | cyclamen | |
| dc.subject.en | Fig mosaic emaravirus | |
| dc.subject.en | high-throughput sequencing | |
| dc.title.en | High-Throughput Sequencing Reveals <em>Cyclamen persicum</em> Mill. as a Natural Host for Fig Mosaic Virus. | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.3390/v10120684 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
| bordeaux.journal | Viruses | |
| bordeaux.page | 684 | |
| bordeaux.volume | 10 | |
| bordeaux.issue | 12 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02621862 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02621862v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Viruses&rft.date=2018&rft.volume=10&rft.issue=12&rft.spage=684&rft.epage=684&rft.eissn=1999-4915&rft.issn=1999-4915&rft.au=ELBEAINO,%20Toufic&MARAIS,%20Armelle&FAURE,%20Chantal&TRIOANO,%20Elisa&CANDRESSE,%20Thierry&rft.genre=article |
Fichier(s) constituant ce document
| Fichiers | Taille | Format | Vue |
|---|---|---|---|
|
Il n'y a pas de fichiers associés à ce document. |
|||