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Cloning and transplantation of the <em>Mesoplasma florum</em> genome.

hal.structure.identifierDépartement de biologie [Sherbrooke] [UdeS]
dc.contributor.authorBABY, Vincent
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLABROUSSAA, Fabien
hal.structure.identifierDépartement de biologie [Sherbrooke] [UdeS]
dc.contributor.authorBRODEUR, Joëlle
hal.structure.identifierDépartement de biologie [Sherbrooke] [UdeS]
dc.contributor.authorMATTEAU, Dominick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Géraldine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierDépartement de biologie [Sherbrooke] [UdeS]
dc.contributor.authorRODRIGUE, Sebastien
dc.date.issued2018
dc.identifier.issn2161-5063
dc.description.abstractEnCloning and transplantation of bacterial genomes is a powerful method for the creation of engineered microorganisms. However, much remains to be understood about the molecular mechanisms and limitations of this approach. We report the whole-genome cloning of Mesoplasma florum in Saccharomyces cerevisiae, and use this model to investigate the impact of a bacterial chromosome in yeast cells. Our results indicate that the cloned M. florum genome is subjected to weak transcriptional activity, and causes no significant impact on yeast growth. We also report that the M. florum genome can be transplanted into Mycoplasma capricolum without any negative impact from the putative restriction enzyme encoding gene mfl307. Using whole-genome sequencing, we observed that a small number of mutations appeared in all M. florum transplants. Mutations also arose, albeit at a lower frequency, when the M. capricolum genome was transplanted into M. capricolum recipient cells. These observations suggest that genome transplantation is mutagenic, and that this phenomenon is magnified by increased phylogenetic distance between the genome donor and the recipient cell. No difference in efficiency was detected after three successive rounds of genome transplantation, suggesting that the observed mutations were not selected during the procedure. Taken together, our results provide a more accurate picture of the events taking place during bacterial genome cloning and transplantation.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectgenome transplantation
dc.subjectSaccharomyces cerevisiae
dc.subject.enwhole'genome cloning
dc.subject.enMesoplasma florum
dc.title.enCloning and transplantation of the <em>Mesoplasma florum</em> genome.
dc.typeArticle de revue
dc.identifier.doi10.1021/acssynbio.7b00279
dc.subject.halSciences du Vivant [q-bio]/Biologie animale
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.journalACS Synthetic Biology
bordeaux.page209-217
bordeaux.volume7
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02623732
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02623732v1
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