Afficher la notice abrégée

NtTPN1: a RPP8‐like R gene required for Potato virus Y‐induced veinal necrosis in tobacco

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMICHEL, Vincent
hal.structure.identifierImperial Tobacco Group
dc.contributor.authorJULIO, Emilie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierImperial Tobacco Group
dc.contributor.authorCOTUCHEAU, Julien
hal.structure.identifierImperial Tobacco Group
dc.contributor.authorDECORPS, Christophe
hal.structure.identifierImperial Tobacco Group
dc.contributor.authorVOLPATTI, Roxane
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorMOURY, Benoît
hal.structure.identifierInstitut de Génétique, Environnement et Protection des Plantes [IGEPP]
hal.structure.identifierFédération Nationale des Producteurs de Plants de Pomme de Terre [FN3PT]
dc.contributor.authorGLAIS, Laurent
hal.structure.identifierImperial Tobacco Group
dc.contributor.authorDORLHAC DE BORNE, François
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROOCQ, Véronique
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
dc.date.issued2018-08
dc.identifier.issn0960-7412
dc.description.abstractEnPotato virus Y is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVYN) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. However, va‐overcoming PVY isolates inducing necrotic symptoms were observed in several countries. In this context, it is important to find va‐independent protection strategies. In a previous study, the phenotyping of 162 tobacco varieties revealed ten accessions that do not carry the va allele and do not exhibit typical PVYN‐induced veinal necrosis. Despite the absence of necrotic symptoms, normal viral accumulation in these plants suggests a va‐independent mechanism of tolerance to PVYN‐induced systemic veinal necrosis. Fine mapping of the genetic determinant(s) was performed in a segregating F2 population. The tolerance trait is inherited as a single recessive gene and allelism tests demonstrated that eight of the ten tolerant varieties carry the same determinant. Anchoring the linkage map to the tobacco genome physical map allowed the identification of a RPP8‐like R gene, called NtTPN1 (for <em>Nicotiana tabacum</em> Tolerance to PVY‐induced Necrosis1), with the same single‐nucleotide polymorphism in the eight tolerant accessions. Functional assays using homozygous NtTPN1 EMS mutants confirmed the role of NtTPN1 in the tolerance phenotype. PVYN‐induced systemic veinal necrosis in tobacco likely represents an inefficient defense response with HR‐like characteristics. The identification of NtTPN1 opens breeding options to minimize the impact of emerging and so far uncontrolled va‐breaking necrotic PVY isolates.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
dc.language.isoen
dc.publisherWiley
dc.subjecttolerance
dc.subjectveinal necrosis
dc.subject.enRPP8-like gene
dc.subject.enPVY
dc.subject.enPotyvirus
dc.subject.enresistance
dc.subject.enHR
dc.subject.enR gene
dc.subject.enNicotiana tabacum
dc.title.enNtTPN1: a RPP8‐like R gene required for Potato virus Y‐induced veinal necrosis in tobacco
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.13980
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalPlant Journal
bordeaux.page700-714
bordeaux.volume95
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02623766
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02623766v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Plant%20Journal&amp;rft.date=2018-08&amp;rft.volume=95&amp;rft.issue=4&amp;rft.spage=700-714&amp;rft.epage=700-714&amp;rft.eissn=0960-7412&amp;rft.issn=0960-7412&amp;rft.au=MICHEL,%20Vincent&amp;JULIO,%20Emilie&amp;CANDRESSE,%20Thierry&amp;COTUCHEAU,%20Julien&amp;DECORPS,%20Christophe&amp;rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée