Afficher la notice abrégée

A novel <em>badnavirus</em> discovered from <em>Betula sp.</em> affected by birch leaf-roll disease.

hal.structure.identifierHumboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin [HU Berlin]
dc.contributor.authorRUMBOU, Artemis
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTHEIL, Sébastien
hal.structure.identifierHumboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin [HU Berlin]
dc.contributor.authorLANGER, Juliane
hal.structure.identifierNatural Resources Institute Finland [LUKE]
dc.contributor.authorJALKANEN, Risto
hal.structure.identifierHumboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin [HU Berlin]
dc.contributor.authorBÜTTNER, Carmen
dc.date.issued2018
dc.identifier.issn1932-6203
dc.description.abstractEnIn declining birches (Betula sp.) from different European stands affected by the "birch leaf-roll disease" (BLRD) a novel virus is identified by means of RNA-Seq virome analysis. The virus represents a new member in the genus Badnavirus, family Caulimoviridae, tentatively named Birch leaf roll-associated virus (BLRaV) and it is the first badnavirus found to infect birch. Complete genome sequences (7,862-7,864 nucleotides) of three viral isolates of Finnish and German origin have been determined. The virus sequences show a typical badnavirus organization with three major open reading frames (ORFs) and a fourth potential ORF overlapping with the end of ORF3. ORFs 1-2-3 show low level of amino acid identity to the corresponding proteins encoded by other badnaviruses, reaching a maximum of 44% identity (ORF3). Grapevine vein-clearing virus appears as the closest badnavirus when considering the polymerase region. So far, we can exclude evidence for presence of endogenous BLRaV elements in the birch genome, while evidence for the episomal activity of BLRaV is provided. The viral population holds significant haplotype diversity, while co-infection by different BLRaV variants are observed in single hosts. BLRaV presence is associated with the BLRD in both silver (B. pendula) and downy birch (B. pubescens). These results challenge the earlier hypothesis of a causal role of Cherry leaf roll virus in BLRD. Further work is now needed to finally prove that BLRaV is the causal agent for the BLRD.
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enbirch leaf-roll disease
dc.subject.enBLRD
dc.subject.enBirch leaf roll-associated virus
dc.title.enA novel <em>badnavirus</em> discovered from <em>Betula sp.</em> affected by birch leaf-roll disease.
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0193888
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalPLoS ONE
bordeaux.pagee0193888
bordeaux.volume13
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02624650
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02624650v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=PLoS%20ONE&amp;rft.date=2018&amp;rft.volume=13&amp;rft.issue=3&amp;rft.spage=e0193888&amp;rft.epage=e0193888&amp;rft.eissn=1932-6203&amp;rft.issn=1932-6203&amp;rft.au=RUMBOU,%20Artemis&amp;CANDRESSE,%20Thierry&amp;MARAIS,%20Armelle&amp;THEIL,%20S%C3%A9bastien&amp;LANGER,%20Juliane&amp;rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée