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QTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.).

hal.structure.identifierCentre for Research in Agricultural Genomics (CRAG)
dc.contributor.authorARGYRIS, Jason M
hal.structure.identifierInstitut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology [IRTA]
dc.contributor.authorDÍAZ, Aurora
hal.structure.identifierCentre for Research in Agricultural Genomics (CRAG)
dc.contributor.authorRUGGIERI, Valentino
hal.structure.identifierSemillas Fitó S.A.
dc.contributor.authorFERNÁNDEZ, Marta
hal.structure.identifierSemillas Fitó S.A.
dc.contributor.authorJAHRMANN, Torben
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierUniversitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia [UPV]
dc.contributor.authorPICÓ, Belén
hal.structure.identifierCentre for Research in Agricultural Genomics (CRAG)
dc.contributor.authorMARTÍN-HERNÁNDEZ, Ana M
hal.structure.identifierUniversitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia [UPV]
dc.contributor.authorMONFORTE, Antonio J
hal.structure.identifierCentre for Research in Agricultural Genomics (CRAG)
dc.contributor.authorGARCIA-MAS, Jordi
dc.date.issued2017
dc.identifier.issn1664-462X
dc.description.abstractEnSugar content is the major determinant of both fruit quality and consumer acceptance in melon (Cucumis melo L), and is a primary target for crop improvement. Near-isogenic lines (NILs) derived from the intraspecific cross between a "Piel de Sapo" (PS) type and the exotic cultivar "Songwhan Charmi" (SC), and several populations generated from the cross of PS × Ames 24294 ("Trigonus"), a wild melon, were used to identify QTL related to sugar and organic acid composition. Seventy-eight QTL were detected across several locations and different years, with three important clusters related to sugar content located on chromosomes 4, 5, and 7. Two PS × SC NILs (SC5-1 and SC5-2) sharing a common genomic interval of 1.7 Mb at the top of chromosome 5 contained QTL reducing soluble solids content (SSC) and sucrose content by an average of 29 and 68%, respectively. This cluster collocated with QTL affecting sugar content identified in other studies in lines developed from the PS × SC cross and supported the presence of a stable consensus locus involved in sugar accumulation that we named SUCQSC5.1. QTL reducing soluble solids and sucrose content identified in the "Trigonus" mapping populations, as well as QTL identified in previous studies from other ssp. agrestis sources, collocated with SUCQSC5.1, suggesting that they may be allelic and implying a role in domestication. In subNILs derived from the PS × SC5-1 cross, SUCQSC5.1 reduced SSC and sucrose content by an average of 18 and 34%, respectively, and was fine-mapped to a 56.1 kb interval containing four genes. Expression analysis of the candidate genes in mature fruit showed differences between the subNILs with PS alleles that were "high" sugar and SC alleles of "low" sugar phenotypes for MELO3C014519, encoding a putative BEL1-like homeodomain protein. Sequence differences in the gene predicted to affect protein function were restricted to SC and other ssp. agrestis cultivar groups. These results provide the basis for further investigation of genes affecting sugar accumulation in melon.
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectNILs
dc.subjectQTL
dc.subject.enBEL1-like
dc.subject.encandidate genes
dc.subject.enfine-mapping
dc.subject.enmelon
dc.subject.ensucrose
dc.subject.ensugar
dc.title.enQTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.).
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2017.01679
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalFrontiers in Plant Science
bordeaux.page1679
bordeaux.volume8
bordeaux.peerReviewedoui
hal.identifierhal-02628388
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02628388v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2017&rft.volume=8&rft.spage=1679&rft.epage=1679&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=ARGYRIS,%20Jason%20M&D%C3%8DAZ,%20Aurora&RUGGIERI,%20Valentino&FERN%C3%81NDEZ,%20Marta&JAHRMANN,%20Torben&rft.genre=article


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