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Transcriptomic Analysis of <em>Prunus domestica</em> Undergoing Hypersensitive Response to <em>Plum Pox Virus</em> Infection

hal.structure.identifierConsejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] [CSIC]
dc.contributor.authorRODAMILANS, Bernardo
hal.structure.identifierConsejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] [CSIC]
dc.contributor.authorSAN LEÓN, David
hal.structure.identifierCenter of Life and Food Sciences Weihenstephan
dc.contributor.authorMÜHLBERGER, Louisa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierCenter of Life and Food Sciences Weihenstephan
dc.contributor.authorNEUMÜLLER, Michael
hal.structure.identifierConsejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] [CSIC]
dc.contributor.authorOLIVEROS, Juan Carlos
hal.structure.identifierConsejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] [CSIC]
dc.contributor.authorGARCÍA, Juan Antonio
dc.date.issued2014
dc.identifier.issn1932-6203
dc.description.abstractEnPlum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named 'Jojo', develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. Analysis of viral reads from the infected samples allowed reconstruction of a PPV-D consensus sequence. De novo reconstruction showed a second viral isolate of the PPV-Rec strain. RNA-seq analysis of PPV-infected 'Jojo' trees identified 2,234 and 786 unigenes that were significantly up- or downregulated, respectively (false discovery rate; FDR≤0.01). Expression of genes associated with defense was generally enhanced, while expression of those related to photosynthesis was repressed. Of the total of 3,020 differentially expressed unigenes, 154 were characterized as potential resistance genes, 10 of which were included in the NBS-LRR type. Given their possible role in plant defense, we selected 75 additional unigenes as candidates for further study. The combination of next-generation sequencing and a Prunus variety that develops a hypersensitive response to PPV infection provided an opportunity to study the factors involved in this plant defense mechanism. Transcriptomic analysis presented an overview of the changes that occur during PPV infection as a whole, and identified candidates suitable for further functional characterization.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enTranscriptomic Analysis of <em>Prunus domestica</em> Undergoing Hypersensitive Response to <em>Plum Pox Virus</em> Infection
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0100477
dc.subject.halSciences du Vivant [q-bio]/Autre [q-bio.OT]
bordeaux.journalPLoS ONE
bordeaux.pagee100477
bordeaux.volume9
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02631444
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02631444v1
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