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Highly dynamic genomic loci drive the synthesis of two types of capsular or secreted polysaccharides within the "Mycoplasma mycoides" cluster.

hal.structure.identifierContrôle des maladies animales exotiques et émergentes [UMR CMAEE]
dc.contributor.authorBERTIN, Clothilde
hal.structure.identifierBiologie des Plantes et Innovation - UR UPJV 3900 [BIOPI]
dc.contributor.authorPAU-ROBLOT, Corinne
hal.structure.identifierBiologie des Plantes et Innovation - UR UPJV 3900 [BIOPI]
dc.contributor.authorCOURTOIS, Josiane
hal.structure.identifierContrôle des maladies animales exotiques et émergentes [UMR CMAEE]
dc.contributor.authorMANSO-SILVÁN, Lucía
hal.structure.identifierUniversité de Lyon
dc.contributor.authorTARDY, Florence
hal.structure.identifierUniversité de Lyon
dc.contributor.authorPOUMARAT, François
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierUniversité de Lyon
dc.contributor.authorGAURIVAUD, Patrice
hal.structure.identifierContrôle des maladies animales exotiques et émergentes [UMR CMAEE]
dc.contributor.authorTHIAUCOURT, François
dc.date.issued2015
dc.identifier.issn0099-2240
dc.description.abstractEnMycoplasmas of the "Mycoplasma mycoides" cluster are all ruminant pathogens. Mycoplasma mycoides subsp. mycoides (MmmSC) is responsible for contagious bovine pleuropneumonia and is known to produce capsular polysaccharide (CPS) and exopolysaccharide (EPS). Previous studies have strongly suggested a role for MmmSC polysaccharides in pathogenicity. MmmSC-secreted EPS was recently characterized as a β-(1-6)- galactofuranose homopolymer (galactan) identical to the capsular product. Here, we extended the characterization of secreted polysaccharides to all other members of the "M. mycoides" cluster: M. capricolum subsp capripneumoniae (Mccp) M. capricolum subsp capricolum (Mcc), M. leachii and M. mycoides subsp capri (Mmc, including the "LC" and "capri" serovars). Extracted EPS were characterized by nuclear magnetic resonance, resulting in the identification of a homopolymer of β-(1-2)-glucopyranose (glucan) in Mccp and M. leachii. Monoclonal antibodies specific for this glucan and for the MmmSC-secreted galactan, were used to detect the two polysaccharides. While Mmc strains of the "LC" serovar produced only capsular galactan, no polysaccharide could be detected in strains of the "capri" serovar. All strains of Mccp and M. leachii produced glucan CPS and EPS, whereas glucan production and localization varied among Mcc strains. Genes associated with polysaccharide synthesis, and forming a biosynthetic pathway, were predicted in all cluster members. These genes were organized in clusters within two loci representing genetic variability hotspots. Phylogenetic analysis showed that some of these genes, notably galE and glf, were acquired via horizontal gene transfer. These findings call for a reassessment of the specificity of the serological tests based on mycoplasma polysaccharides.
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.subject.enMollicutes
dc.subject.enMycoplasma mycoides
dc.subject.enRuminant
dc.subject.enAnimal Diseases
dc.subject.enPathogenic bacteria
dc.title.enHighly dynamic genomic loci drive the synthesis of two types of capsular or secreted polysaccharides within the "Mycoplasma mycoides" cluster.
dc.typeArticle de revue
dc.identifier.doi10.1128/AEM.02892-14
dc.subject.halSciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animal
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
bordeaux.journalApplied and Environmental Microbiology
bordeaux.page676-687
bordeaux.volume81
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02635944
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02635944v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.date=2015&rft.volume=81&rft.issue=2&rft.spage=676-687&rft.epage=676-687&rft.eissn=0099-2240&rft.issn=0099-2240&rft.au=BERTIN,%20Clothilde&PAU-ROBLOT,%20Corinne&COURTOIS,%20Josiane&MANSO-SILV%C3%81N,%20Luc%C3%ADa&TARDY,%20Florence&rft.genre=article


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