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The Glycerol-3-Phosphate Acyltransferase GPAT6 from Tomato Plays a Central Role in Fruit Cutin Biosynthesis.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPETIT, Johann
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorWONG JUN TAI, Virginie
hal.structure.identifierCornell University [New York]
dc.contributor.authorMARTIN, Laetitia B B
hal.structure.identifierCornell University [New York]
dc.contributor.authorFICH, Eric A
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorJOUBES, Jerome
hal.structure.identifierCornell University [New York]
dc.contributor.authorROSE, Jocelyn K C
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorDOMERGUE, Frederic
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2016
dc.identifier.issn0032-0889
dc.description.abstractEnThe thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6). To this end, a backcross population (BC1F2) segregating for the glossy trait was phenotyped. Individuals displaying either a wild-type or a glossy fruit trait were then pooled into bulked populations and submitted to whole-genome sequencing prior to mutation frequency analysis. This revealed that the causal point mutation in the gpat6-a mutant introduces a charged amino acid adjacent to the active site of a GPAT6 enzyme. We further showed that this mutation completely abolished the GPAT activity of the recombinant protein. The gpat6-a mutant showed perturbed pollen formation but, unlike a gpat6 mutant of Arabidopsis (Arabidopsis thaliana), was not male sterile. The most striking phenotype was observed in the mutant fruit, where cuticle thickness, composition, and properties were altered. RNA sequencing analysis highlighted the main processes and pathways that were affected by the mutation at the transcriptional level, which included those associated with lipid, secondary metabolite, and cell wall biosynthesis.
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.title.enThe Glycerol-3-Phosphate Acyltransferase GPAT6 from Tomato Plays a Central Role in Fruit Cutin Biosynthesis.
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.16.00409
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Physiology
bordeaux.page894-913
bordeaux.volume171
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02637507
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02637507v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Physiology&rft.date=2016&rft.volume=171&rft.issue=2&rft.spage=894-913&rft.epage=894-913&rft.eissn=0032-0889&rft.issn=0032-0889&rft.au=PETIT,%20Johann&BRES,%20C%C3%A9cile&MAUXION,%20Jean-Philippe&WONG%20JUN%20TAI,%20Virginie&MARTIN,%20Laetitia%20B%20B&rft.genre=article


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