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Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening.

hal.structure.identifierHangzhou Normal University
dc.contributor.authorCHEN, Weiwei
hal.structure.identifierHangzhou Normal University
dc.contributor.authorKONG, Junhua
hal.structure.identifierHangzhou Normal University
dc.contributor.authorQIN, Cheng
hal.structure.identifierThe Chinese University of Hong Kong [Hong Kong] [CUHK]
dc.contributor.authorYU, Sheng
hal.structure.identifierHangzhou Normal University
dc.contributor.authorTAN, Jinjuan
hal.structure.identifierThe Chinese University of Hong Kong [Hong Kong] [CUHK]
dc.contributor.authorCHEN, Yun-Ru
hal.structure.identifierHangzhou Normal University
dc.contributor.authorWU, Chaoqun
hal.structure.identifierHangzhou Normal University
dc.contributor.authorWANG, Hui
hal.structure.identifierSchool of Life Sciences
dc.contributor.authorSHI, Yan
hal.structure.identifierSchool of Life Sciences
dc.contributor.authorLI, Chunyang
hal.structure.identifierHangzhou Normal University
dc.contributor.authorLI, Bin
hal.structure.identifierHangzhou Normal University
dc.contributor.authorZHANG, Pengcheng
hal.structure.identifierHangzhou Normal University
dc.contributor.authorWANG, Ying
hal.structure.identifierHangzhou Normal University
dc.contributor.authorLAI, Tongfei
hal.structure.identifierHangzhou Normal University
dc.contributor.authorYU, Zhiming
hal.structure.identifierHangzhou Normal University
dc.contributor.authorZHANG, Xian
hal.structure.identifierHangzhou Normal University
dc.contributor.authorSHI, Nongnong
hal.structure.identifierHangzhou Normal University
dc.contributor.authorWANG, Huizhong
hal.structure.identifierSchool of Life Sciences
dc.contributor.authorOSMAN, Toba
hal.structure.identifierMOE Key Laboratory of Bioinformatics, Centre for Plant Biology, School of Life Sciences
dc.contributor.authorLIU, Yule
hal.structure.identifierSchool of Life Sciences
dc.contributor.authorMANNING, Kenneth
hal.structure.identifierSchool of Life Sciences
dc.contributor.authorJACKSON, Stephen
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROLIN, Dominique
hal.structure.identifierThe Chinese University of Hong Kong [Hong Kong] [CUHK]
dc.contributor.authorZHONG, Silin
hal.structure.identifierUniversity of Nottingham, UK [UON]
dc.contributor.authorSEYMOUR, Graham B
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGALLUSCI, Philippe
hal.structure.identifierSchool of Life Sciences
hal.structure.identifierHangzhou Normal University
dc.contributor.authorHONG, Yiguo
dc.date.issued2015
dc.identifier.issn2045-2322
dc.description.abstractEnNaturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks and how they are inherited remain unknown. Here we report that CHROMOMETHYLASE3 (SlCMT3) and other methyltransferases are required for maintenance of a spontaneous epimutation and its cognate Colourless non-ripening (Cnr) phenotype in tomato. We screened a series of DNA methylation-related genes that could rescue the hypermethylated Cnr mutant. Silencing of the developmentally-regulated SlCMT3 gene results in increased expression of LeSPL-CNR, the gene encodes the SBP-box transcription factor residing at the Cnr locus and triggers Cnr fruits to ripen normally. Expression of other key ripening-genes was also up-regulated. Targeted and whole-genome bisulfite sequencing showed that the induced ripening of Cnr fruits is associated with reduction of methylation at CHG sites in a 286-bp region of the LeSPL-CNR promoter, and a decrease of DNA methylation in differentially-methylated regions associated with the LeMADS-RIN binding sites. Our results indicate that there is likely a concerted effect of different methyltransferases at the Cnr locus and the plant-specific SlCMT3 is essential for sustaining Cnr epi-allele. Maintenance of DNA methylation dynamics is critical for the somatic stability of Cnr epimutation and for the inheritance of tomato non-ripening phenotype.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enRequirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening.
dc.typeArticle de revue
dc.identifier.doi10.1038/srep09192
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalScientific Reports
bordeaux.page9192
bordeaux.volume5
bordeaux.peerReviewedoui
hal.identifierhal-02637773
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02637773v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2015&rft.volume=5&rft.spage=9192&rft.epage=9192&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=CHEN,%20Weiwei&KONG,%20Junhua&QIN,%20Cheng&YU,%20Sheng&TAN,%20Jinjuan&rft.genre=article


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