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Identification of the carotenoid modifying gene PALE YELLOW PETAL 1 as an essential factor in xanthophyll esterification and yellow flower pigmentation in tomato (Solanum lycopersicum).

hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorARIIZUMI, Tohru
hal.structure.identifierNARO Institute of Floricultural Science
dc.contributor.authorKISHIMOTO, Sanae
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorKAKAMI, Ryo
hal.structure.identifierResearch Institute for Production Development
dc.contributor.authorMAOKA, Takashi
hal.structure.identifierKazusa DNA Research Institute [KDRI]
dc.contributor.authorHIRAKAWA, Hideki
hal.structure.identifierThe University of Tokyo [UTokyo]
dc.contributor.authorSUZUKI, Yutaka
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOZEKI, Yuko
hal.structure.identifierKazusa DNA Research Institute [KDRI]
dc.contributor.authorSHIRASAWA, Kenta
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOKABE, Yoshihiro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierKazusa DNA Research Institute [KDRI]
dc.contributor.authorASAMIZU, Erika
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierNARO Institute of Floricultural Science
dc.contributor.authorOHMIYA, Akemi
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
dc.date.issued2014
dc.identifier.issn0960-7412
dc.description.abstractEnXanthophylls, the pigments responsible for yellow to red coloration, are naturally occurring carotenoid compounds in many colored tissues of plants. These pigments are esterified within the chromoplast; however, little is known about the mechanisms underlying their accumulation in flower organs. In this study, we characterized two allelic tomato (Solanum lycopersicum L.) mutants, pale yellow petal (pyp) 1-1 and pyp1-2, that have reduced yellow color intensity in the petals and anthers due to loss-of-function mutations. Carotenoid analyses showed that the yellow flower organs of wild-type tomato contained high levels of xanthophylls that largely consisted of neoxanthin and violaxanthin esterified with myristic and/or palmitic acids. Functional disruption of PYP1 resulted in loss of xanthophyll esters, which was associated with a reduction in the total carotenoid content and disruption of normal chromoplast development. These findings suggest that xanthophyll esterification promotes the sequestration of carotenoids in the chromoplast and that accumulation of these esters is important for normal chromoplast development. Next-generation sequencing coupled with map-based positional cloning identified the mutant alleles responsible for the pyp1 phenotype. PYP1 most likely encodes a carotenoid modifying protein that plays a vital role in the production of xanthophyll esters in tomato anthers and petals. Our results provide insight into the molecular mechanism underlying the production of xanthophyll esters in higher plants, thereby shedding light on a longstanding mystery.
dc.language.isoen
dc.publisherWiley
dc.subjectplastoglobule
dc.subject.enSolanum lycopersicum
dc.subject.enCarotenoids
dc.subject.enchromoplast
dc.subject.enesterification
dc.subject.engene cloning
dc.subject.enxanthophyll
dc.title.enIdentification of the carotenoid modifying gene PALE YELLOW PETAL 1 as an essential factor in xanthophyll esterification and yellow flower pigmentation in tomato (Solanum lycopersicum).
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.12570
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalThe Plant Journal
bordeaux.page453-465
bordeaux.volume79
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02639514
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02639514v1
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