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Chromosomal transfers in mycoplasmas: when minimal genomes go mobile

hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDORDET FRISONI, Emilie
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorSAGNÉ, Eveline
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorBARANOWSKI, Eric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRETON, Marc
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorNOUVEL, Laurent Xavier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierUniversity of Melbourne
hal.structure.identifierMelbourne Veterinary School [Parkville, VIC, Australie] [MVS]
dc.contributor.authorMARENDA, Marc Serge
hal.structure.identifierMycoplasmoses des Ruminants [MYCO]
dc.contributor.authorTARDY, Florence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
dc.date.issued2014-11
dc.identifier.issn2161-2129
dc.description.abstractEnHorizontal gene transfers (HGT) shape bacterial genomes and are key contributors to microbial diversity and innovation. One main mechanism involves conjugation, a process that allows the simultaneous transfer of significant amounts of DNA upon cell-to-cell contact. Recognizing and deciphering conjugal mechanisms are thus essential in understanding the impact of gene flux on bacterial evolution. We addressed this issue in mycoplasmas, the smallest and simplest self-replicating bacteria. In these organisms, HGT was long thought to be marginal. We showed here that nearly every position of the Mycoplasma agalactiae chromosome could be transferred via conjugation, using an unconventional mechanism. The transfer involved DNA blocks containing up to 80 genes that were incorporated into the host chromosome by homologous recombination. These findings radically change our views concerning mycoplasma evolution and adaptation with particularly far-reaching implications given that over 50 species are human or animal pathogens.IMPORTANCE: Horizontal gene transfers (HGT) shape bacterial genomes and are key contributors to microbial diversity and innovation. One main mechanism involves conjugation, a process that allows the simultaneous transfer of significant amounts of DNA upon cell-to-cell contact. Recognizing and deciphering conjugal mechanisms are thus essential in understanding the impact of gene flux on bacterial evolution. We addressed this issue in mycoplasmas, the smallest and simplest self-replicating bacteria. In these organisms, HGT was long thought to be marginal. We showed here that nearly every position of the Mycoplasma agalactiae chromosome could be transferred via conjugation, using an unconventional mechanism. The transfer involved DNA blocks containing up to 80 genes that were incorporated into the host chromosome by homologous recombination. These findings radically change our views concerning mycoplasma evolution and adaptation with particularly far-reaching implications given that over 50 species are human or animal pathogens.
dc.description.sponsorshipEchange de gènes entre pathogènes " minimaux " par transfert horizontal : mécanismes et impacts sur l'évolution, la virulence et l'adaptation à l'hôte - ANR-09-MIEN-0016
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.title.enChromosomal transfers in mycoplasmas: when minimal genomes go mobile
dc.typeArticle de revue
dc.identifier.doi10.1128/mBio.01958-14
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalmBio
bordeaux.pagee01958-14
bordeaux.volume5
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02640054
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640054v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=mBio&rft.date=2014-11&rft.volume=5&rft.issue=6&rft.spage=e01958-14&rft.epage=e01958-14&rft.eissn=2161-2129&rft.issn=2161-2129&rft.au=DORDET%20FRISONI,%20Emilie&SAGN%C3%89,%20Eveline&BARANOWSKI,%20Eric&BRETON,%20Marc&NOUVEL,%20Laurent%20Xavier&rft.genre=article


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