Mostrar el registro sencillo del ítem
In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | TSARMPOPOULOS, Iason | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GOURGUES, Geraldine | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BLANCHARD, Alain | |
hal.structure.identifier | J. Craig Venter Institute [La Jolla, USA] [JCVI] | |
dc.contributor.author | VASHEE, Sanjay | |
hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
dc.contributor.author | JORES, Joerg | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | LARTIGUE, Carole | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | SIRAND-PUGNET, Pascal | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2161-5063 | |
dc.description.abstractEn | One remarkable achievement in synthetic biology was the reconstruction of mycoplasma genomes and their cloning in yeast where they can be modified using available genetic tools. Recently, CRISPR/Cas9 editing tools were developed for yeast mutagenesis. Here, we report their adaptation for the engineering of bacterial genomes cloned in yeast. A seamless deletion of the mycoplasma glycerol-3-phosphate oxidase-encoding gene (glpO) was achieved without selection in one step, using 90 nt paired oligonucleotides as templates to drive recombination. Screening of the resulting clones revealed that more than 20% contained the desired deletion. After manipulation, the overall integrity of the cloned mycoplasma genome was verified by multiplex PCR and PFGE. Finally, the edited genome was back-transplanted into a mycoplasma recipient cell. In accordance with the deletion of glpO, the mutant mycoplasma was affected in the production of H2O2. This work paves the way to high-throughput manipulation of natural or synthetic genomes in yeast. | |
dc.language.iso | en | |
dc.publisher | American Chemical Society | |
dc.subject | CRISPR/Cas9 | |
dc.subject | Saccharomyces cerevisiae | |
dc.subject | genome transplantation | |
dc.subject.en | Mycoplasma | |
dc.subject.en | genome engineering | |
dc.subject.en | seamless gene deletion | |
dc.title.en | In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9 | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1021/acssynbio.5b00196 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
bordeaux.journal | ACS Synthetic Biology | |
bordeaux.page | 104-109 | |
bordeaux.volume | 5 | |
bordeaux.issue | 1 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02640111 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02640111v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Synthetic%20Biology&rft.date=2016&rft.volume=5&rft.issue=1&rft.spage=104-109&rft.epage=104-109&rft.eissn=2161-5063&rft.issn=2161-5063&rft.au=TSARMPOPOULOS,%20Iason&GOURGUES,%20Geraldine&BLANCHARD,%20Alain&VASHEE,%20Sanjay&JORES,%20Joerg&rft.genre=article |
Archivos en el ítem
Archivos | Tamaño | Formato | Ver |
---|---|---|---|
No hay archivos asociados a este ítem. |