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Metabolomic profiling in tomato reveals diel compositional changes in fruit affected by source-sink relationships.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoit
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorOSORIO, Sonia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickael
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALLIAS, Patricia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIE, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCABASSON, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJACOB, Daniel
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorVERCAMBRE, Gilles
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorGAUTIER, Hélène
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROLIN, Dominique
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorGÉNARD, Michel
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorFERNIE, Alisdair R
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
dc.date.issued2015
dc.identifier.issn0022-0957
dc.description.abstractEnA detailed study of the diurnal compositional changes was performed in tomato (Solanum lycopersicum cv. Moneymaker) leaves and fruits. Plants were cultivated in a commercial greenhouse under two growth conditions: control and shaded. Expanding fruits and the closest mature leaves were harvested during two different day/night cycles (cloudy or sunny day). High-throughput robotized biochemical phenotyping of major compounds, as well as proton nuclear magnetic resonance and mass spectrometry metabolomic profiling, were used to measure the contents of about 70 metabolites in the leaves and 60 metabolites in the fruits, in parallel with ecophysiological measurements. Metabolite data were processed using multivariate, univariate, or clustering analyses and correlation networks. The shaded carbon-limited plants adjusted their leaf area, decreased their sink carbon demand and showed subtle compositional modifications. For source leaves, several metabolites varied along a diel cycle, including those directly linked to photosynthesis and photorespiration. These metabolites peaked at midday in both conditions and diel cycles as expected. However, transitory carbon storage was limited in tomato leaves. In fruits, fewer metabolites showed diel fluctuations, which were also of lower amplitude. Several organic acids were among the fluctuating metabolites. Diel patterns observed in leaves and especially in fruits differed between the cloudy and sunny days, and between the two conditions. Relationships between compositional changes in leaves and fruits are in agreement with the fact that several metabolic processes of the fruit appeared linked to its momentary supply of sucrose.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectfruit metabolism
dc.subjectmetabolomics
dc.subject.en1H-NMR
dc.subject.enDiurnal changes
dc.subject.enMS
dc.subject.enSolanum lycopersicum.
dc.title.enMetabolomic profiling in tomato reveals diel compositional changes in fruit affected by source-sink relationships.
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erv151
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Experimental Botany
bordeaux.page3391-404
bordeaux.volume66
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-02641303
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02641303v1
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