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Specific membrane lipid composition is important for Plasmodesmata function in Arabidopsis

hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGRISON, Magali S.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierInstitut National de la Recherche Agronomique [INRA]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorBROCARD, Lysiane
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorFOUILLEN, Laetitia
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorNICOLAS, William
hal.structure.identifierInstitute of Molecular Physiology and Biotechnology of Plants
dc.contributor.authorWEWER, Vera
hal.structure.identifierInstitute of Molecular Physiology and Biotechnology of Plants
dc.contributor.authorDÖRMANN, Peter
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorNACIR, Houda
hal.structure.identifierUniversity of Leeds
dc.contributor.authorBENITEZ-ALFONSO, Yoselin
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGERMAIN, Véronique
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBOUTTÉ, Yohann
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorMONGRAND, Sébastien
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAYER, Emmanuelle M.
dc.date.issued2015
dc.identifier.issn1040-4651
dc.description.abstractEnPlasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made in identifying PD proteins, the role played by major membrane constituents, such as the lipids, in defining specialized membrane domains in PD remains unknown. Through a rigorous isolation of "native" PD membrane fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids are laterally segregated along the plasma membrane (PM) at the PD cell-to-cell junction in Arabidopsis thaliana. Remarkably, our results show that PD membranes display enrichment in sterols and sphingolipids with very long chain saturated fatty acids when compared with the bulk of the PM. Intriguingly, this lipid profile is reminiscent of detergent-insoluble membrane microdomains, although our approach is valuably detergent-free. Modulation of the overall sterol composition of young dividing cells reversibly impaired the PD localization of the glycosylphosphatidylinositol-anchored proteins Plasmodesmata Callose Binding 1 and the beta-1,3-glucanase PdBG2 and altered callose-mediated PD permeability. Altogether, this study not only provides a comprehensive analysis of the lipid constituents of PD but also identifies a role for sterols in modulating cell-to-cell connectivity, possibly by establishing and maintaining the positional specificity of callose-modifying glycosylphosphatidylinositol proteins at PD. Our work emphasizes the importance of lipids in defining PD membranes.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.title.enSpecific membrane lipid composition is important for Plasmodesmata function in Arabidopsis
dc.typeArticle de revue
dc.identifier.doi10.1105/tpc.114.135731
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences de l'environnement
bordeaux.journalThe Plant cell
bordeaux.page1228-1250
bordeaux.volume27
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02641388
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02641388v1
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