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hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSVANELLA-DUMAS, Laurence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierInstitut de Génétique, Environnement et Protection des Plantes [IGEPP]
dc.contributor.authorHULLÉ, Maurice
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS-COLOMBEL, Armelle
dc.date.issued2013
dc.identifier.issn1932-6203
dc.description.abstractEnA systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its prevalence in P. cookii were analyzed using samples collected from various sites of the archipelago. We estimate the average prevalence of BYDV to be 24.9% in P. cookii, with significant variability between sites. BYDV genetic diversity was assessed using sequence information from two genomic regions: the P3 open reading frame (ORF) (encoding the coat protein) and the hypervariable P6 ORF region. The phylogenetic analysis in the P3 region showed that BYDV sequences segregate into three major lineages, the most frequent of which (Ker-I cluster) showed close homology with BYDV-PAV-I isolates and had very low intra-lineage diversity (0.6%). A similarly low diversity was also recorded in the hypervariable P6 region, suggesting that Ker-I isolates derive from the recent introduction of BYDV-PAV-I. Divergence time estimation suggests that BYDV-PAV-I was likely introduced in the Kerguelen environment at the same time frame as its aphid vector, Rhopalosiphum padi, whose distribution shows good overlap with that of BYDV-Ker-I. The two other lineages show more than 22% amino acid divergence in the P3 region with other known species in the BYDV species complex, indicating that they represent distinct BYDV species. Using species-specific amplification primers, the distribution of these novel species was analyzed. The high prevalence of BYDV on native Poaceae and the presence of the vector R. padi, raises the question of its impact on the vulnerable plant communities of this remote ecosystem.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enDistribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0067231
dc.subject.halSciences du Vivant [q-bio]/Autre [q-bio.OT]
bordeaux.journalPLoS ONE
bordeaux.pagee67231
bordeaux.volume8
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-01208609
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01208609v1
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