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Sequence variability, recombination analysis, and specific detection of the W strain of Plum pox virus

hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorGLASA, Miroslav
hal.structure.identifierResearch Institute of Pomology and Floriculture
dc.contributor.authorMALINOWSKI, Tadeusz
hal.structure.identifierSlovak Academy of Sciences [SAS]
dc.contributor.authorPREDAJŇA, Lukáš
hal.structure.identifierState Institute of Fruit-Growing
dc.contributor.authorPUPOLA, Neda
hal.structure.identifierPure Horticultural Research Centre
dc.contributor.authorDEKENA, Dzintra
hal.structure.identifierResearch Institute of Pomology and Floriculture
dc.contributor.authorMICHALCZUK, Lech
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2011
dc.identifier.issn0031-949X
dc.description.abstractEnPlum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of Sharka, the most detrimental disease of stone-fruit trees worldwide. PPV isolates are grouped into seven distinct strains. The minor PPV-W strain was established recently for the divergent W3174 isolate found in Canada. Here, the partial or complete genomic sequences of four PPV-W isolates from Latvia have been determined. The completely sequenced isolates LV-141pl and LV-145bt share 93.1 and 92.1% nucleotide identity, respectively, with isolate W3174, with two regions of higher (>20%) divergence in the P1/HC-Pro and NIa (VPg) regions. Further analyses demonstrated that these two regions correspond to two independent recombination events in the W3174 genome, one involving PPV-M (approximate genome positions 692 to 1424) and the other PPV-D (nucleotides 5672 to 5789). The LV-141pl and LV-145bt isolates appear to be representatives of the “ancestral” PPV-W strain, not affected by recombination. The PPV-W intrastrain variability is substantially higher than that of all other PPV strains, with potential implications for the serological detection of PPV-W isolates. A PPV-W-specific primer pair has been developed, allowing the specific reverse-transcription polymerase chain reaction detection of all five presently available W isolates. The characterization of these new PPV-W isolates sheds light on PPV-W evolutionary history, further supports the hypothesis of its East-European origin, and opens the way for the biological and epidemiological characterization of this poorly known PPV strain.
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.subjectvirologie
dc.subjectcoat protein
dc.subjectppv
dc.subjectpopulations
dc.subjectevolution
dc.subjectdna
dc.subject.enwinona
dc.subject.enisolat
dc.subject.endiversity
dc.subject.ennucleotide sequence
dc.subject.ensharka disease
dc.subject.enrna viruses
dc.subject.enpotyvirus
dc.subject.enisolate
dc.title.enSequence variability, recombination analysis, and specific detection of the W strain of Plum pox virus
dc.typeArticle de revue
dc.identifier.doi10.1094/PHYTO-12-10-0334
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalPhytopathology
bordeaux.page980-985
bordeaux.volume101
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-02647282
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647282v1
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