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Enhanced polyamine accumulation alters carotenoid metabolism at the transcriptional level in tomato fruit over-expressing spermidine synthase

hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorNEILY, Mohamed Hichem
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorMATSUKURA, Chiaki
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickael
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorYIN, Yong-Gen
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorSAITO, Takeshi
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorMORI, Kentaro
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorASAMIZU, Erika
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROLIN, Dominique
hal.structure.identifierNational Institute of Fruit Sciences
dc.contributor.authorMORIGUCHI, Takaya
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
dc.date.issued2011
dc.identifier.issn0176-1617
dc.description.abstractEnPolyamines are involved in crucial plant physiological events, but their roles in fruit development remain unclear. We generated transgenic tomato plants that show a 1.5- to 2-fold increase in polyamine content by over-expressing the spermidine synthase gene, which encodes a key enzyme for polyamine biosynthesis. Pericarp-columella and placental tissue from transgenic tomato fruits were subjected to (1)H-nuclear magnetic resonance (NMR) for untargeted metabolic profiling and high-performance liquid chromatography-diode array detection for carotenoid profiling to determine the effects of high levels of polyamine accumulation on tomato fruit metabolism. A principal component analysis of the quantitative (1)H NMR data from immature green to red ripe fruit showed a clear discrimination between developmental stages, especially during ripening. Quantification of 37 metabolites in pericarp-columella and 41 metabolites in placenta tissues revealed distinct metabolic profiles between the wild type and transgenic lines, particularly at the late ripening stages. Notably, the transgenic tomato fruits also showed an increase in carotenoid accumulation, especially in lycopene (1.3- to 2.2-fold), and increased ethylene production (1.2- to 1.6-fold) compared to wild-type fruits. Genes responsible for lycopene biosynthesis, including phytoene synthase, phytoene desaturase, and deoxy-d-xylulose 5-phosphate synthase, were significantly up-regulated in ripe transgenic fruits, whereas genes involved in lycopene degradation, including lycopene-epsilon cyclase and lycopene beta cyclase, were down-regulated in the transgenic fruits compared to the wild type. These results suggest that a high level of accumulation of polyamines in the tomato regulates the steady-state level of transcription of genes responsible for the lycopene metabolic pathway, which results in a higher accumulation of lycopene in the fruit.
dc.language.isoen
dc.publisherElsevier
dc.subjectBIOLOGIE DU DEVELOPPEMENT
dc.subjectBIOLOGIE MOLECULAIRE
dc.subjectBIOLOGIE VEGETALE
dc.subject.enCAROTENOID BIOSYNTHESIS
dc.subject.enMETABOLIC PROFILING
dc.subject.enPOLYAMINES
dc.subject.enSPERMIDINE SYNTHASE
dc.title.enEnhanced polyamine accumulation alters carotenoid metabolism at the transcriptional level in tomato fruit over-expressing spermidine synthase
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jplph.2010.07.003
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalJournal of Plant Physiology
bordeaux.page242-252
bordeaux.volume168
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02647454
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647454v1
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