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Tomato GDSL1 is required for cutin deposition in the fruit cuticle

hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorGIRARD, Anne-Laure
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorMOUNET, Fabien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE-CHAMLEY, Martine
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorGAILLARD, Cedric
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorELMORJANI, Khalil
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorVIVANCOS, Julien
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorRUNAVOT, Jean-Luc
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorQUEMENER, Bernard
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPETIT, Johann
hal.structure.identifierStation de physiologie végétale
dc.contributor.authorGERMAIN, Veronique
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorMARION, Didier
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorBAKAN, Benedicte
dc.date.issued2012
dc.identifier.issn1040-4651
dc.description.abstractEnThe plant cuticle consists of cutin, a polyester of glycerol, hydroxyl, and epoxy fatty acids, covered and filled by waxes. While the biosynthesis of cutin building blocks is well documented, the mechanisms underlining their extracellular deposition remain unknown. Among the proteins extracted from dewaxed tomato (Solanum lycopersicum) peels, we identified GDSL1, a member of the GDSL esterase/acylhydrolase family of plant proteins. GDSL1 is strongly expressed in the epidermis of growing fruit. In GDSL1-silenced tomato lines, we observed a significant reduction in fruit cuticle thickness and a decrease in cutin monomer content proportional to the level of GDSL1 silencing. A significant decrease of wax load was observed only for cuticles of the severely silenced transgenic line. Fourier transform infrared (FTIR) analysis of isolated cutins revealed a reduction in cutin density in silenced lines. Indeed, FTIR-attenuated total reflectance spectroscopy and atomic force microscopy imaging showed that drastic GDSL1 silencing leads to a reduction in ester bond cross-links and to the appearance of nanopores in tomato cutins. Furthermore, immunolabeling experiments attested that GDSL1 is essentially entrapped in the cuticle proper and cuticle layer. These results suggest that GDSL1 is specifically involved in the extracellular deposition of the cutin polyester in the tomato fruit cuticle.
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.title.enTomato GDSL1 is required for cutin deposition in the fruit cuticle
dc.typeArticle de revue
dc.identifier.doi10.1105/tpc.112.101055
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
bordeaux.journalThe Plant cell
bordeaux.page3119-3134
bordeaux.volume24
bordeaux.issue7
bordeaux.peerReviewedoui
hal.identifierhal-02650850
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02650850v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=The%20Plant%20cell&rft.date=2012&rft.volume=24&rft.issue=7&rft.spage=3119-3134&rft.epage=3119-3134&rft.eissn=1040-4651&rft.issn=1040-4651&rft.au=GIRARD,%20Anne-Laure&MOUNET,%20Fabien&LEMAIRE-CHAMLEY,%20Martine&GAILLARD,%20Cedric&ELMORJANI,%20Khalil&rft.genre=article


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