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Plant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During the Medicago truncatula–Sinorhizobium meliloti Symbiosis

hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierUniversity of Florida [Gainesville] [UF]
dc.contributor.authorBERGER, Antoine
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorBOSCARI, Alexandre
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorHORTA ARAÚJO, Natasha
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickaël
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorHANCHI, Mohamed
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorROLIN, Dominique
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorPUPPO, Alain
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorBROUQUISSE, Renaud
dc.date.issued2020
dc.identifier.issn1664-462X
dc.description.abstractEnNitrate reductase (NR) is the first enzyme of the nitrogen reduction pathway in plants, leading to the production of ammonia. However, in the nitrogen-fixing symbiosis between legumes and rhizobia, atmospheric nitrogen (N 2) is directly reduced to ammonia by the bacterial nitrogenase, which questions the role of NR in symbiosis. Next to that, NR is the best-characterized source of nitric oxide (NO) in plants, and NO is known to be produced during the symbiosis. In the present study, we first surveyed the three NR genes (MtNR1, MtNR2, and MtNR3) present in the Medicago truncatula genome and addressed their expression, activity, and potential involvement in NO production during the symbiosis between M. truncatula and Sinorhizobium meliloti. Our results show that MtNR1 and MtNR2 gene expression and activity are correlated with NO production throughout the symbiotic process and that MtNR1 is particularly involved in NO production in mature nodules. Moreover, NRs are involved together with the mitochondrial electron transfer chain in NO production throughout the symbiotic process and energy regeneration in N 2-fixing nodules. Using an in vivo NMR spectrometric approach, we show that, in mature nodules, NRs participate also in the regulation of energy state, cytosolic pH, carbon and nitrogen metabolism under both normoxia and hypoxia. These data point to the importance of NR activity for the N 2-fixing symbiosis and provide a first explanation of its role in this process.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.description.sponsorshipMécanismes contrôlant la transition entre fixation d'azote et sénescence dans les nodosités symbiotiques de légumineuses - ANR-15-CE20-0005
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectMétabolisme
dc.subject.enhypoxia
dc.subject.enlegumes
dc.subject.enMedicago truncatula
dc.subject.ennitric oxide
dc.subject.ennitrogen-fixing symbiosis
dc.subject.ennitrate reductase
dc.subject.ennodules
dc.title.enPlant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During the Medicago truncatula–Sinorhizobium meliloti Symbiosis
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2020.01313
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
bordeaux.journalFrontiers in Plant Science
bordeaux.page1313
bordeaux.volume11
bordeaux.peerReviewedoui
hal.identifierhal-02983822
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02983822v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2020&rft.volume=11&rft.spage=1313&rft.epage=1313&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=BERGER,%20Antoine&BOSCARI,%20Alexandre&HORTA%20ARA%C3%9AJO,%20Natasha&MAUCOURT,%20Micka%C3%ABl&HANCHI,%20Mohamed&rft.genre=article


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