Mostrar el registro sencillo del ítem
A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses
hal.structure.identifier | National Institute of Biology [Ljubljana] [NIB] | |
dc.contributor.author | KUTNJAK, Denis | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | TAMISIER, Lucie | |
hal.structure.identifier | Fera Science Limited | |
dc.contributor.author | ADAMS, Ian | |
hal.structure.identifier | Newcastle University [Newcastle] | |
dc.contributor.author | BOONHAM, Neil | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | CANDRESSE, Thierry | |
hal.structure.identifier | CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP] | |
dc.contributor.author | CHIUMENTI, Michela | |
hal.structure.identifier | Research Institute for Agricultural, Fisheries and Food [ILVO] | |
dc.contributor.author | DE JONGHE, Kris | |
hal.structure.identifier | International Potato Center [Lima] [CIP] | |
dc.contributor.author | KREUZE, Jan F. | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | LEFEBVRE, Marie | |
hal.structure.identifier | Natural Resources Institute (NRI), University of Greenwich, UK | |
dc.contributor.author | SILVA, Goncalo | |
hal.structure.identifier | United States Department of Agriculture - Animal and Plant Health Inspection Service | |
dc.contributor.author | MALAPI-WIGHT, Martha | |
hal.structure.identifier | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH / Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures [DSMZ] | |
dc.contributor.author | MARGARIA, Paolo | |
hal.structure.identifier | University of Maribor | |
dc.contributor.author | MAVRIC PLESCO, Irena | |
hal.structure.identifier | Fera Science Limited | |
dc.contributor.author | MCGREIG, Sam | |
hal.structure.identifier | CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP] | |
dc.contributor.author | MIOZZI, Laura | |
hal.structure.identifier | Laboratoire de santé des végétaux [LSV Angers] | |
dc.contributor.author | REMENANT, Benoit | |
hal.structure.identifier | Agroscope | |
dc.contributor.author | REYNARD, Jean Sebastien | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
hal.structure.identifier | DNA Vision SA | |
dc.contributor.author | ROLLIN, Johan | |
hal.structure.identifier | Canadian Food Inspection Agency [CFIA] | |
dc.contributor.author | ROTT, Mike | |
hal.structure.identifier | Agroscope | |
dc.contributor.author | SCHUMPP, Olivier | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | MASSART, Sébastien | |
hal.structure.identifier | Research Institute for Agricultural, Fisheries and Food [ILVO] | |
dc.contributor.author | HAEGEMAN, Annelies | |
dc.date.issued | 2021-04-14 | |
dc.identifier.issn | 2076-2607 | |
dc.description.abstractEn | High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of generated sequences, it is of utmost importance that researchers can rely on efficient and reliable bioinformatic tools and can understand the principles, advantages, and disadvantages of the tools used. Here, we present a critical overview of the steps involved in HTS as employed for plant virus detection and virome characterization. We start from sample preparation and nucleic acid extraction as appropriate to the chosen HTS strategy, which is followed by basic data analysis requirements, an extensive overview of the in-depth data processing options, and taxonomic classification of viral sequences detected. By presenting the bioinformatic tools and a detailed overview of the consecutive steps that can be used to implement a well-structured HTS data analysis in an easy and accessible way, this paper is targeted at both beginners and expert scientists engaging in HTS plant virome projects | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.rights.uri | http://creativecommons.org/licenses/by/ | |
dc.subject | Pathologie Végétale | |
dc.subject | Virologie végétale | |
dc.subject | Santé des plantes | |
dc.subject.en | plant virus | |
dc.subject.en | high-throughput sequencing | |
dc.subject.en | bioinformatics | |
dc.subject.en | detection | |
dc.subject.en | discovery | |
dc.title.en | A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses | |
dc.type | Article de revue | |
dc.type | Article de synthèse | |
dc.identifier.doi | 10.3390/microorganisms9040841 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
bordeaux.journal | Microorganisms | |
bordeaux.page | 841 | |
bordeaux.volume | 9 | |
bordeaux.issue | 4 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-03200602 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-03200602v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microorganisms&rft.date=2021-04-14&rft.volume=9&rft.issue=4&rft.spage=841&rft.epage=841&rft.eissn=2076-2607&rft.issn=2076-2607&rft.au=KUTNJAK,%20Denis&TAMISIER,%20Lucie&ADAMS,%20Ian&BOONHAM,%20Neil&CANDRESSE,%20Thierry&rft.genre=article&unknown |
Archivos en el ítem
Archivos | Tamaño | Formato | Ver |
---|---|---|---|
No hay archivos asociados a este ítem. |